BsmB I 

Origin:
Bacillus stearothermophilus B61

Concentration:
10 u/µl

Recognition site:
CGTCTC(N)₁/
GCAGAG(N)₅/

Reaction buffer:
GM-buffer III, (50 mM Tris-HCl 【pH 7.9 @ 25°C】,100 mM NaCl, 10 mM MgCl₂, 100 μg/ml BSA)

Storage conditions:
10 mM Tris-HCl (pH 7.4 @ 25°C), 300 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 500/ml BSA, 50% glycerol.

Storage temperature:
-20°C

Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 10-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16℃ for 16 hr. Of the ligation products, more than 95% can be re-cut.

Optimum temperature:
55°C

Heat inactivation: 80°C for 20 min.

Enzyme activity in % of maximum: