BsiW I (PspL I, Spl I)

Origin:
Bacillus species

Concentration:
10 u/µl

Recognition site:
C/GTACG
GCATG/C

Reaction buffer:
GM-buffer III, (50 mM Tris-HCl 【pH 7.9 @ 25°C】, 100 mM NaCl, 10 mM MgCl₂, 100 μg/ml BSA)

Storage conditions:
10 mM Tris-HCl (pH 7.4 @ 25°C), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 μg/ml BSA and 50% glycerol.

Storage temperature:
-20°C

Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 10-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut.

Optimum temperature: 55°C

Inactivation 20 minutes: 80°C for 20 min.

Enzyme activity in % of maximum: