Bgl I
Origin:
Bacillus globigii
Concentration:
5 u/µl
Recognition site:
GCCNNNN/NGGC
CGGN/NNNNCCG
Reaction buffer:
GM-buffer III , (50 mM Tris-HCl 【pH 7.9 @ 25°C】,100 mM NaCl, 10 mM MgCl2, 100 μg/ml BSA)
Storage conditions:
20 mM Tris-HCl (pH 7.5 @ 25°C), 200 mM NaCl, 0.1 mM EDTA, 10 mM 2-mercaptoethanol, 200 μg/ml BSA, 50% glycerol.
Storage temperature:
-20°C
Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 50-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut.
Optimum temperature:
37°C
Heat inactivation: 65°C for 20 min.
Enzyme activity in % of maximum:
| I |
II |
III |
IV |
ONE |
| 75 |
75 |
100 |
50 |
100 |
| Product (停產) |
| Cat. No. |
Size |
| SZ016S |
200 U |
| SZ016M |
1000 U |
| SZ016L |
5000 U |
  
| Product |
| Cat. No. |
Size |
| E025S |
500 U |
| E025 |
1000 U |
| E026 |
5000 U |
|
