Bal I

Origin:
Brevibacterium albidum

Concentration:
5 u/µl

Recognition site:
TGG/CCA
ACC/GGT

Reaction buffer:
GM-buffer Bal I, (50 mM Tris-HCl【pH 8.2 @25°C】, 5 mM MgCl₂)

Storage conditions:
10 mM Tris-HCl (pH 7.4 @ 25°C), 50 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 500 μg/ml BSA, 50% glycerol.

Storage temperature:  -20°C

Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 20-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut.

Optimum temperature: 37°C

Heat inactivation: 65°C for 20 min.

Enzyme activity in % of maximum: