Ava II (Bme18 I, Eco47 I)

Origin:
Anabaena variabilis

Concentration:
10 u/µl

Recognition site:
G/GWCC
CCWG/G

Reaction buffer:
GM-buffer IV, (20 mM Tris-acetate 【pH 7.9 @ 25°C】, 50 mM potassium acetate, 10 mM magnesium acetate, 100 μg/ml BSA)

Storage conditions:
10 mM Tris-HCl 【pH 7.4 @ 25°C】, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 μg/ml BSA and 50% glycerol.

Storage temperature: -20°C

Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 50-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut. 

Optimum temperature: 37°C

Heat inactivation: 80°C for 20 min.

Enzyme activity in % of maximum: