Apa I

Origin:
Acetobacter pasteurianus

Concentration:
10 u/µl

Recognition site:
GGGCC/C
C/CCGGG

Reaction buffer:
GM-buffer IV, (20 mM Tris-acetate【pH 7.9 @ 25°C】,50 mM potassium acetate, 10 mM magnesium acetate, 100 μg/ml BSA) 

Storage conditions:
10 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 7 mM 2-mercaptoethanol, 200 μg/ml BSA, 50% glycerol

Storage temperature: -20°C

Ligation:
After 10-fold overdigestion with Apa I, >90% of λ DNA fragments can be ligated and recut.

Non-specific hydrolysis: 

No nonspecific activity was detected after incubation of 1 μg of λ DNA with 20 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature: 25°C

Heat inactivation: 65°C for 20 min.

Enzyme activity in % of maximum: