Alu I

Origin:
E. coli strain that carries the cloned Alu I gene from Arthrobacter luteus

Concentration:
2000 - 5000 u.a./ml

Recognition site:
AG/CT
TC/GA

Reaction buffer:
GM-buffer Y, (33mM Tris-acetate(pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM poatassium acetate; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol

Storage temperature: -20°C

Ligation:
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature: 37°C

Inactivation 20 minutes: under 65°C

Enzyme activity in % of maximum: