Ahd I (Dri I, NruG I)

Origin:
Deinococcus radiodurans EA

Concentration:
5000 - 10000, u.a./ml

Recognition site:
GACNNN/NNGTC
CTGNN/NNNCAG

Reaction buffer:
GM-buffer Y , (33 mM Tris-acetate(pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM poatassium acetate;

Storage conditions:
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol

Storage temperature: -20°C

Ligation:
After 5-fold overdigestion with enzyme more than 5% of the DNA fragments can be ligated. Ligation is better in presence of 10% PEG.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature: 37°C

Inactivation 20 minutes: under 65°C

Enzyme activity in % of maximum: