Acc65 I

Origin:
Acinetobacter calcoaceticus 65

Concentration:
10000 - 30000, u.a./ml

Recognition site:
C/GTACC
CCATG/G

Reaction buffer:
GM-buffer W, (10 mM Tris-acetate (pH 8.5 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol

Storage temperature:  -20°C

Ligation:
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 30 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:  37°C

Inactivation 20 minutes: under 65°C

Enzyme activity in GM buffer by %: