Acc I (Fbl I)

Origin:
Acinetobacter calcoaceticus

Concentration:
4 u/µl

Recognition site:
GT/MKAC
CAKM/TG

Reaction buffer:
GM-buffer IV, (20 mM Tris-acetate【pH 7.9 @ 25°C】, 50 mM potassium acetate, 10 mM magnesium acetate, 100 μg/ml BSA)

Storage conditions:
10 mM Tris-HCl (pH 7.5 @ 25°C), 100 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.15% Triton, 200 μg/ml BSA, 50% glycerol.

Storage temperature: -20°C

Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 50-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut.

Optimum temperature:  37°C

Heat inactivation: 80°C for 20 min.

Enzyme activity in GM buffer by %: