人成骨肉瘤细胞核提取物 货号：E-36025
Saos-2 nuclear extract
背景资料：Saos-2 nuclear extract was prepared from cell cultures of the human sarcoma osteogenic Saos-2 cell line. This non-transformed cell line was derived from the primary osteosarcoma of an 11-year old Caucasian female patient. Saos-2 cells exhibit many characteristic osteoblastic properties, including high levels of alkaline phosphatase expression, parathyroid hormone-mediated induction of adenylate cyclase production, and production and secretion of type I collagen and mineralized matrix. Therefore, they are commonly used to study osteoblastic phenotype-related cellular functions and products and in research related to late-stage osteoblastic differentiation.
产品描述：
Saos-2 nuclear extract is supplied in 20 mM Hepes pH 7.5, 400 mM NaCl, 20% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 µM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM β-glycerophosphate, 1 mM DTT and protease inhibitors. The protein content has been determined by a Bradford-based assay.
产品应用：
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
产品使用：
Human osteosarcoma cells
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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