蛋白抑制剂 货号：E-37491
Protease Inhibitor Cocktail
背景资料：Active Motif's Protease Inhibitor Cocktail is designed to prevent proteolytic degradation during lysis and extraction procedures from mammalian cells or tissues. The cocktail contains a broad-spectrum of inhibitors targeting cysteine proteases, serine proteases, aspartic acid proteases, and aminopeptidases. In addition to being ready-to-use, the Protease Inhibitor cocktail is also EDTA-free. The cocktail is supplied as a 100X stock solution in a liquid format that has been stabilized in dimethylsulfoxide (DMSO) for improved accuracy, solubility, and ease of use in comparison to traditional tablets. To see the complete formulation, please click on the Description tab below.
产品描述：
The Protease Inhibitor Cocktail is a broad-spectrum solution of protease inhibitors specific for cysteine, serine, and acid proteases, as well as, aminopeptidases. It has been optimized and tested for use with mammalian cell and tissue extract samples. It is recommended to use 10 µl of the Protease Inhibitor Cocktail solution to inhibit digestion of proteins for 1 ml of lysate. The cocktail does not contain phosphatase inhibitors. If necessary, phosphatase inhibitors can be added to cell and tissue lysates. Since protease levels may vary among cell and tissue types, it may be necessary to increase the concentration of inhibitors.
产品应用：
Broad-spectrum inhibition of proteolysis
Optimized for use with mammalian cell and tissue extracts
Convenient and stable liquid format for ease of use
产品使用：
Formulation and targets:AEBSF, 104 mM - serine proteasesAprotinin, 80 µM - serine proteasesBestatin, 4 mM - aminopeptidasesE-64, 1.4 mM - cysteine proteasesLeupeptin, 2 mM - serine and cysteine proteasesPepstatin A, 1.5 mM - aspartic acid proteases
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
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