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Epigenetics |
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| Epigenetics>【Zymo】 |
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Anti-5-Methylcytosine (A3001-50 A3001-200) |
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Product Type: Monoclonal Antibody |
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Format: Purified |
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Clone: 10G4 |
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Source: Mouse |
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Isotype: IgG1 |
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Specificity: 5-Methylcytosine in single stranded DNA from any source including human, rat, mouse, plants, etc. and 5-Methylcytidine |
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Approximate Protein Concentration: 1 mg/ml |
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Buffer Solution: Antibody is provided in PBS (Phosphate Buffered Saline, pH 7.4) with 0.01% Thimerosal as a preservative. |
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Storage: Store at 0-4°C with prolonged storage at -80°C. Avoid repeated freeze/thawing. |
The ability to detect and quantify DNA methylation (i.e., 5-methylcytosine) efficiently and accurately has become essential for the study of cancer, gene expression, genetic diseases, as well as many other important aspects of epigenetics.
The mouse Anti-5-Methylcytosine monoclonal antibody (clone 10G4) has been developed to facilitate differentiation between methylated and non-methylated cytosines in DNA. Specificity of this clone is to 5-methylcytosines in single stranded DNA with no detectable cross-reactivity to non-methylated cytosines. The antibody has proven to be a valuable tool in the characterization of DNA methylation and has been successfully used for immunopreciptiation-based assays such as Methylated DNA Immunoprecipitation (MeIP).
Methylated DNA is efficiently enriched using the 5-Methylcytosine antibody. DNA was immunoprecipitated using the mouse Anti-5-Methylcytosine 10G4 antibody from a mixed methylated/non-methylated DNA population. Methylated DNA can be cut with Nco I whereas non-methylated DNA is resistant to Nco I digestion. The DNA (post-IP) was subsequently amplified by PCR and digested with Nco I. Products were then separated in a 2.0% (w/v) agarose/TAE/EtBr gel. The image above demonstrates specific enrichment of methylated versus non-methylated DNA by the Anti-5-Methylcytosine 10G4 antibody.
Application and Usage Information:
| Application |
Used |
Recommended Dilution |
| ELISA Application |
Yes |
1:500 - 1:1,000 |
| Flow Cytometry |
N/D ** |
N/A * |
| Immunoblotting |
Yes |
N/A * |
| Immunofluorescence |
Yes |
N/A * |
| Immunohistology |
N/D ** |
N/A * |
| Immunoprecipitation (IP) of Methylated DNA |
Yes |
2-4 µg per IP |
| Western Blotting |
N/D ** |
N/A * |
* N/A = Data not available. ** N/D = Not determined.
| Product |
Catalog No. |
Size |
Price(RMB) |
Protocol |
| Anti-5-Methylcytosine (1 µg/µl) |
A3001-50 |
50 µg |
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A3001-200 |
200 µg |
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