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Epigenetics

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Epigenetics>【Zymo】

	Anti-5-Hydroxymethylcytosine Polyclonal Antibody (E-A4001)

Anti-5-Hydroxymethylcytosine Polyclonal Antibody 货号：E-A4001

背景资料：Recent advances in the field of epigenetics have identified 5-hydroxymethylcytosine (5-hmC) as a key factor in the regulation of gene expression, with substantial implications in the study of tissue differentiation, neurological development, and carcinogenesis. Studies of this epigenetic marker are typically confounded by a lack of reliable methodology for differentiation from the highly prevalent 5-methylcytosine in a DNA sample. The rabbit Anti-5-hydroxymethylcytosine polyclonal antibody has been developed in order to robustly distinguish between hydroxymethylated DNA and methylated or unmodified DNA. Specificity of the antibody is enhanced such that crossreactivity with unmodified and methylated templates is suppressed to near-background levels. The antibody has been extensively tested and validated in ELISA and immunoprecipitation-based enrichment assays, and is suitable for use in further applications including immunohistochemical labeling and chromatographic blotting.

产品描述：Low cross reactivity with Cytosine and 5-methylcytosine versus other available antibodies.

Can be used with a variety of genomic DNA sources.

High sensitivity to low masses of 5-Hydroxymethylcytosine DNA.

产品特点：

5-hmC Glucosyltransferase	5-hmC Glucosyltransferase is guaranteed for 1 year at -20°C. Long term storage at -80°C is recommended. Avoid multiple freeze thawing.
Quest 5-hmC Control DNA	A 90 bp DNA standard used for qPCR. [10 ng/μl] in storage buffer (10mM Tris-HCl; 0.1mM EDTA; pH 8.0) supplied in 30 μl volume. See Appendix I (pg. 6) in Protocol for more details.
Quest qPCR Primers	Control primers specific to Quest 5-hmC Control DNA validated for qPCR. See Appendix II (pgs. 7, 8) in Protocol for detailed information.

保存建议：请在接收到Anti-5-Hydroxymethylcytosine Polyclonal Antibody 产品后，按照产品操作说明书对试剂盒内不同组份进行适当的保存。

定购信息：

产品名称	规格	操作手册	货号	询价
Anti-5-Hydroxymethylcytosine Polyclonal Antibody	25 µg/25 μl	PDF	E-A4001-025
Anti-5-Hydroxymethylcytosine Polyclonal Antibody	50 μg/50 μl	PDF	E-A4001-050
Anti-5-Hydroxymethylcytosine Polyclonal Antibody	200 μg/200 μl	PDF	E-A4001-200

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化试剂盒(定性分析)	48 次	PDF	A-P-1011-48
通用DNA甲基化试剂盒(定性分析)	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

推荐阅读：

关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶.