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  RNAlater®-ICE Frozen Tissue Transition Solution (A-AM7030)
   

RNAlater®-ICE Frozen Tissue Transition Solution                                                                     货号:A-AM7030

RNAlater®-ICE Frozen Tissue Transition Solution

背景资料:A novel reagent for transitioning frozen tissue to a state enabling easy extraction of high-quality RNA; frozen tissues are simply submerged in Ambion® RNAlater®-ICE and allowed to thaw overnight at –20°C. It is provided in one bottle containing 25 mL. Once thawed, the tissues can be processed like fresh tissues using standard RNA isolation procedures。

产品描述:

• Process previously frozen tissues like freshly harvested samples
• Thawing tissues in RNAlater®-ICE protects RNA from degradation
• No more tissue pulverizing with mortar and pestle and awkward transfer of powder to tube
• Easily apportion frozen tissue samples for multiple experiments。

产品特点:

Quick Freezing Tissues Preserves RNA;Often, tissues that need to be stored prior to RNA isolation are "snap" or "flash" frozen on dry ice or in liquid nitrogen to preserve RNA integrity. RNA in tissue is stable while frozen at –80°C, but thawing the tissue for RNA purification can result in RNA degradation. This is true even if the tissue thaws while in the denaturation solution.

Processing Frozen Tissues is Problematic;Frozen tissues are typically ground with a chilled mortar and pestle in order to maintain RNA quality. Liquid nitrogen must be added to the mortar to keep the sample frozen while it is ground. Powdered tissue can also thaw during transfer to a homogenization vessel. This often results in formation of clumps that do not readily disperse in the lysis solution, resulting in RNA degradation and loss.

Process Frozen Tissue Without Jeopardizing RNA Integrity;RNAlater®-ICE solves all of these problems. Simply submerge frozen tissue samples in 10 volumes of the solution and store overnight at –20°C (the solution will remain as liquid at these temperatures). As the tissue thaws, RNA integrity is protected. Once treated, tissue can be safely stored at 4°C or even at room temperature (for a limited period of time) and can be further dissected or processed prior to homogenization in a standard RNA isolation lysis buffer. Thus the same frozen tissue sample can be used multiple times for different experiments without compromising RNA integrity.

保存建议:在接收到艾德寄出的试剂盒后请按照说明书建议,使用不同的保存条件或温度来保存试剂盒内组分

定购信息:

产品名称 规格 操作手册 货号 询价
RNAlater®-ICE Frozen Tissue Transition Solution 25 ml PDF A-AM7030  点击这里给我发消息
RNAlater® RNA Stabilization Solution 100 ml PDF A-AM7020  
RNAlater® RNA Stabilization Solution 500 ml PDF A-AM7021  
动物组织样本RNA长期保存与纯化液 5 次 PDF A-RS214-00  
动物组织样本RNA长期保存与纯化液 50 次 PDF A-RS214-01  
动物组织样本RNA长期保存与纯化液 200 次 PDF A-RS214-02  
微生物样本直接PCR试剂盒 100 次 PDF A-M313-01  
微生物样本直接PCR试剂盒 500 次 PDF A-M313-02  
全血/血清直接PCR试剂盒 50 次 PDF A-P7605-50  
全血/血清直接PCR试剂盒 200 次 PDF A-P7605-200  

表观遗传学产品全面解决方案列下:

产品名称 规格 操作手册 货号 询价
DNA羟甲基化定量试剂盒(比色法) 48 次 PDF A-P-1035-48 点击这里给我发消息
DNA羟甲基化定量试剂盒(比色法) 96 次 PDF A-P-1035-96  
DNA甲基化定量试剂盒(荧光法) 48 次 PDF A-P-1036-48 点击这里给我发消息
DNA甲基化定量试剂盒(荧光法) 96 次 PDF A-P-1036-96  
DNA甲基化定量试剂盒(比色法) 48 次 PDF A-P-1034-48 点击这里给我发消息
DNA甲基化定量试剂盒(比色法) 96 次 PDF A-P-1034-96  
通用DNA甲基化定量试剂盒 48 次 PDF A-P-1011-48 点击这里给我发消息
通用DNA甲基化定量试剂盒 96 次 PDF A-P-1011-96  
超敏DNA甲基化定量试剂盒 48 次 PDF A-P-1021-48 点击这里给我发消息
超敏DNA甲基化定量试剂盒 96 次 PDF A-P-1021-96  
总DNA甲基化定量试剂盒 48 次 PDF A-P-1014-48 点击这里给我发消息
总DNA甲基化定量试剂盒 96 次 PDF A-P-1014-96  

推荐阅读:

关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。

 
 
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