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  GenoMatrix 全基因组扩增试剂盒(A-8001)
   

GenoMatrix™ 全基因组扩增试剂盒                                                   货号:A-8001

GenoMatrix™ Whole Genome Amplification Kit

背景资料:

Whole Genome Amplification without any representational bias

Whole Genome Amplification (WGA) is used to amplify an entire genome from a small amount of genomic DNA, so any gene in the organism can be studied while preserving the limited sample. The GenoMatrix™ Whole Genome Amplification Kit provides up to a 500-fold amplification starting from as little as 10 ng of any type of genomic DNA, while maintaining sequence representation of the starting material. GenoMatrix Kits provide all reagents needed to quickly and easily perform whole genome amplification. The kit was designed for high-efficiency, genome-representative amplification, and is validated for use with samples generated by ChIP-IT® Express and the MethylCollector™ & HypoMethylCollector™ Kits, which enables seamless transition into microarray analysis or other applications that require large quantities of DNA.

产品描述:

What is the GenoMatrix Whole Genome Amplification Kit?

The GenoMatrix Whole Genome Amplification Kit provides all the reagents and buffers required to quickly and easily perform a genome-representative amplification of any type of genomic DNA. In particular, the method works especially well with ChIP samples and samples from MethylCollector™ or HypoMethylCollector™ experiments. All the reagents and enzymes provided in GenoMatrix have been optimized for the highest efficiency amplification. The GenoMatrix Kit includes sufficient reagents to perform 50 whole genome amplification reactions.

Preserve the sequence representation of your starting material

Active Motif’s GenoMatrix Whole Genome Amplification Kit utilizes a new approach that virtually eliminates amplification bias, so the amplified material has the same sequence representation as the material that you started with.

Quick and simple

The entire whole genome amplification procedure can be completed in less than two and half hours.

Versatile

The GenoMatrix Whole Genome Amplification Kit can be used as a stand-alone product to amplify unsheared DNA, or with pre-sheared samples generated through use of Active Motif’s ChIP-IT Express, MethylCollector or HypoMethylCollector Kits.

产品特点:

1·Achieve bias-free amplification of genomic DNA samples;

2·Preserve the original sequence representation of your starting material;

3·Obtain enough DNA for downstream applications;

4·Quick and Simple – includes all buffers and reagents needed for whole genome amplification

5·Versatile – can be used as a stand alone product to amplify unsheared DNA, or with pre-sheared samples generated by Active Motif's ChIP-IT Express, MethylCollector and HypoMethylCollector Kits;

保存建议:在接收到艾德寄出的试剂盒后请按照说明书建议,使用不同的保存条件或温度来保存试剂盒内组分

10X Shearing Buffer, GenoMatrix Primer A, GenoMatrix Buffer 1, GenoMatrix Buffer 2, GenoMatrix DNA polymerase, GenoMatrix Primer B, 10X PCR Buffer, Taq DNA polymerase, dNTPs and Human Genomic Control DNA. All reagents should be stored at -20°C. All reagents are guaranteed stable for 6 months when stored properly.

定购信息:

产品名称 规格 操作手册 货号 询价
GenoMatrix™ 全基因组扩增试剂盒 50 次 PDF A-8001-50 点击这里给我发消息
GenoMatrix™ 全基因组扩增试剂盒 100次(2*50次) PDF A-8001-100  
GenoMatrix™ 全基因组扩增试剂盒 200次(4*50次) PDF A-8001-200  

表观遗传学产品全面解决方案列下:

产品名称 规格 操作手册 货号 询价
DNA羟甲基化定量试剂盒(荧光法) 48 次 PDF A-P-1037-48 点击这里给我发消息
DNA羟甲基化定量试剂盒(荧光法) 96 次 PDF A-P-1037-96  
DNA羟甲基化定量试剂盒(比色法) 48 次 PDF A-P-1036-48 点击这里给我发消息
DNA羟甲基化定量试剂盒(比色法) 96 次 PDF A-P-1036-96  
DNA甲基化定量试剂盒(荧光法) 48 次 PDF A-P-1035-48 点击这里给我发消息
DNA甲基化定量试剂盒(荧光法) 96 次 PDF A-P-1035-96  
DNA甲基化定量试剂盒(比色法) 48 次 PDF A-P-1034-48 点击这里给我发消息
DNA甲基化定量试剂盒(比色法) 96 次 PDF A-P-1034-96  
通用DNA甲基化定量试剂盒 48 次 PDF A-P-1011-48 点击这里给我发消息
通用DNA甲基化定量试剂盒 96 次 PDF A-P-1011-96  
超敏DNA甲基化定量试剂盒 48 次 PDF A-P-1021-48 点击这里给我发消息
超敏DNA甲基化定量试剂盒 96 次 PDF A-P-1021-96  
总DNA甲基化定量试剂盒 48 次 PDF A-P-1014-48 点击这里给我发消息
总DNA甲基化定量试剂盒 96 次 PDF A-P-1014-96  

推荐阅读:

关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。

 
 
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