RNA甲基化修饰试剂盒                                                                                               货号：A-P-9003

Methylamp RNA Bisulfite Conversion Kit

背景资料：5-methylcytosine (5-mC) in DNA been well studied and is generally associated with repression of gene expression, but it has also been observed that in humans, 5-mC occurs in various RNA molecules including tRNAs, rRNAs, mRNAs, and non-coding RNAs (ncRNAs). At least 10,275 5-mC candidate sites were discovered in mRNAs and ncRNAs, which covered 10.6% of the total cytosine residues in the transcriptome. 5-mC seems to be enriched in some classes of ncRNA, but relatively depleted in mRNAs. However, the majority (83%) of their candidate sites were found in mRNAs. Within these transcripts, 5-mC appears to be depleted within protein coding sequences but enriched in 5’ and 3’ UTRs. Two different methyltransferases, NSUN2 and DNMT2 are known to catalyze the 5-mC modification in eukaryotic RNAs. Recent data strongly suggest that RNA cytosine methylation affects the regulation of various biological processes such as RNA stability and mRNA translation. Furthermore, loss of 5-mC in vault RNAs causes aberrant processing into Argonaute-associated small RNA fragments that can function as microRNAs. Thus, impaired processing of vault ncRNA may contribute to the etiology of human disorders related to NSUN2-deficiency human disorders. Bisulfite conversion of RNA, followed by RT-PCR amplification, cloning, and sequencing yields reliable information about RNA cytosine methylation states. By treating RNA with bisulfite, cytosine residues are deaminated to uracil while leaving 5-methylcytosine intact.

产品描述：The Methylamp RNA Bisulfite Conversion Kit contains all reagents required for fast and efificient bisulfite conversion on a RNA sample. A unique conversion mix solution contains powerful RNA protection reagents to prevent chemical and thermophilic degradation, thus leading to an accelerated conversion of all cytosines to uracil with negligible methylcytosine deamination. The non-toxic RNA capture solution enables RNA to tightly bind to the column filter, so that converted RNA cleaning can be carried out on the column to effectively remove residual bisulfite and salts。  

产品特点：

1·Fast and convenient protocol that can be finished in 3 hours. ；

2·Completely converts unmethylated RNA cytosine into uracil (>99.9%) with no or negligible inappropriate/error conversion of methylcytosine to thymine (<0.1%) when the indicated range of input sample RNA is used；

3· Powerful protection against RNA degradation, with over 90% of RNA loss prevented；

4.Included control primers are specific against bisulfite-converted RNA and can be used to test whether the bisulfite conversion has been properly achieved；

5.Low amount of input RNA can be used for bisulfite conversion with as low as 5 ng per reactionability ；

6.Simple, reliable, and consistent reaction conditions。

引物信息【试剂盒提供】：

1. The primers are specific to the bisulfite-converted human 28S rRNA 

2.The sequences are as follows: 

28S-F: TTTTAAGTAGGAGGTGTT  

28S-R: ATCTAAACCCAACTCACA

定购信息：

产品名称	规格	操作手册	货号	询价
RNA甲基化修饰试剂盒	50 次	英文PDF--中文PDF	A-P-9003-050
RNA甲基化修饰试剂盒	100 次	英文PDF--中文PDF	A-P-9003-100
RNA甲基化修饰试剂盒	200 次	英文PDF--中文PDF	A-P-9003-200
亚硫酸盐快速DNA文库制备试剂盒(illumina)	12 次	PDF	A-P-1055-12
亚硫酸盐快速DNA文库制备试剂盒(illumina)	24 次	PDF	A-P-1055-24
高敏感亚硫酸盐测序试剂盒(illumina)	12 次	PDF	A-P-1056-12
高敏感亚硫酸盐测序试剂盒(illumina)	24 次	PDF	A-P-1056-24
SETDB1特异基因敲除定量试剂盒-表观遗传调控因子	96 次	PDF	A-P-5002-SETDB1-96
通用乙酰化组蛋白H3K36定量分析试剂盒(荧光法)	96 次	PDF	A-P-4019-96

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化试剂盒(定性分析)	48 次	PDF	A-P-1011-48
通用DNA甲基化试剂盒(定性分析)	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

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关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。