亚硫酸盐快速DNA文库制备试剂盒(与illumina仪器配合使用)                                          货号：A-P-1055

EpiNext Post-Bisulfite DNA Library Preparation Kit (Illumina)

背景资料：Several methods, including whole genome bisulfite sequencing (WGBS) and reduced representation bisulfite sequencing (RRBS), are currently used for genome-wide DNA methlyation analysis. These methods convert unmethylated cytosines to uracil while 5-methylcytosines remain unchanged by the bisulfite treatment. This allows epigenetic differences to become genetic differences, which can be subsequently detected via sequencing at the single-based resolution level and on a genome-wide scale. However, practical use with such current methods are not ideal as they (1) need large amounts of DNA (>1 µg) as input material, which is difficult to prepare from limited biological samples such as tumor biopsy, early embyros, embyronic tissues, and circulating DNA; (2) require DNA to first be sheared and then ligated to adapters, followed by bisulfite conversion (post-ligation bisulfite conversion), which causes substantial amounts of DNA fragments ontained in the adapter-DNA fragment constructs to be broken and thereby forms mono-tagged templates that become removed during library enrichment; and (3) are time-consuming (2 days). The EpiNext Post-Bisulfite DNA Library Preparation Kit is designed to overcome these weaknesses.

产品描述：With this kit, bisulfite-treated DNA (which is in single-stranded form), is converted to double-stranded DNA and directly used for ligation with BisDNA-specific adapters which are necessary for amplification and sequencing. The fragments are then size selected and purified with MQ beads, which allows for quick and precise size selections of DNA. Size-selected DNA fragments are then amplified with a high-fidelity PCR Mix, ensuring maximum yields from minimum amounts of starting material and providing a highly accurate amplification of library DNA with low error rates and minimal bias。  

产品特点：

1·Allows bisulfite-converted DNA to be used directly for ligation, thereby eliminating the possibility of breaking adapter-ligated fragments, which can often occur in currently used WGBS and RRBS methods；

2·Fast 5-hour procedure, from input starting material to library amplification；

3· Gel-free size selection/purification saves time and prevents handling errors, as well as loss of valuable samples；

High sensitivty and efficiency -- direct ligation of adapter to bisulfite-converted DNA fragments reduces loss of fragments and selection bias, which enables pre-bisulfite input DNA to be as low as 1 ng ；

4.Comprehensive set of components to accommodate each step of DNA library preparation -- ligation, clean-up, size selection, and library amplification -- for convenience, consistency, and reliability ；

5.Ultra HiFi amplification enables achievement of reproducibly high yields of DNA libraries with minimal sequence bias and low error rates。

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分

定购信息：

产品名称	规格	操作手册	货号	询价
亚硫酸盐快速DNA文库制备试剂盒(illumina)	12 次	PDF	A-P-1055-12
亚硫酸盐快速DNA文库制备试剂盒(illumina)	24 次	PDF	A-P-1055-24
高敏感亚硫酸盐测序试剂盒(illumina)	12 次	PDF	A-P-1056-12
高敏感亚硫酸盐测序试剂盒(illumina)	24 次	PDF	A-P-1056-24
SETDB1特异基因敲除定量试剂盒-表观遗传调控因子	96 次	PDF	A-P-5002-SETDB1-96
通用乙酰化组蛋白H3K36定量分析试剂盒(荧光法)	96 次	PDF	A-P-4019-96

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化试剂盒(定性分析)	48 次	PDF	A-P-1011-48
通用DNA甲基化试剂盒(定性分析)	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

推荐阅读：

关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。