【艾维缔®A&D Technology Corporation查货热线+86-0313-5935521/520EPIGENETICDNARNAPROTEINMethPrimer

精彩产品
表观遗传学..热销..
分子生物学...A...
生物信息学...B...
细胞生物学...C...
蛋白质组学...D...
免疫生物学...E...
实验试剂学...F...
实验耗材学...G...
实验仪器学...H...
化工原料学...K...
其它生物学...Z...
精彩产品>表观遗传学..热销..
 
  甲酰基(5-fC)定量检测试剂盒(荧光法)(A-P-1042)
   

甲酰基(5-fC)定量检测试剂盒(荧光法)                                                                                           货号:A-P-1042

MethylFlash 5-Formylcytosine (5-fC) DNA Quantification Kit (Fluorometric)

产品背景:The MethylFlash™ 5-Formylcytosine (5-fC) DNA Quantification Kit (Fluorometric) is a complete set of optimized buffers and reagents to colorimetrically quantify 5-formylcytosine (5-fC) in a microplate-based format. It is suitable for use with DNA isolated from any species (mammals, plants, fungi, bacteria, viruses, etc.) in a variety of forms including, but not limited to, cultured cells, fresh and frozen tissues, paraffin-embedded tissues, and body fluid samples.

Background Information
5-formylcytosine (5-fC) has recently been found in mammalian tissue and cells and has been classified as a seventh DNA base. It is formed by the oxidation of 5-hydroxymethylcytosine through TET hydroxylases and has been demonstrated to be an intermediate of the DNA demethylation process. The function of 5-fC in gene regulation is not yet entirely clear, but it has been shown that 5-fC exhibits replication-dependent dilution during mouse pre-implantation development and could be functional in regulating pre-implantation development as a whole. The detection of 5-fC in various tissues and cells is important because 5-fC could be a marker to indicate active DNA demethylation. 5-fC can also be directly excised by Thymine DNA glycosylase (TDG) to allow subsequent base excision repair (BER) processing which converts modified cytosine back to its unmodified state.

The kit has the following advantages and features:

Fast and easy procedure completed within just 3 hours and 45 minutes.

High sensitivity, of which the detection limit can be as low as 1 pg of 5-fC. High specificity with detection of only 5-fC.

No cross-reactivity to cytosine, 5-mC, 5-hmC, or 5-caC within the indicated concentration range of the sample DNA.

Highly convenient assay that uses DNA isolated from cells or tissues directly; no need for DNA digestion or hydrolysis.

保存建议:在接收到艾德寄出的试剂盒后请按照说明书建议,使用不同的保存条件或温度来保存试剂盒内组分

定购信息:

产品名称 规格 操作手册 货号 询价
甲酰基(5-fC)定量检测试剂盒(荧光法) 48 次 PDF A-P-1042-48 点击这里给我发消息
甲酰基(5-fC)定量检测试剂盒(荧光法) 96 次 PDF A-P-1042-96 点击这里给我发消息

表观遗传学产品全面解决方案列下:

产品名称 规格 操作手册 货号 询价
DNA羟甲基化定量试剂盒(荧光法) 48 次 PDF A-P-1037-48 点击这里给我发消息
DNA羟甲基化定量试剂盒(荧光法) 96 次 PDF A-P-1037-48  
DNA羟甲基化定量试剂盒(比色法) 48 次 PDF A-P-1036-48 点击这里给我发消息
DNA羟甲基化定量试剂盒(比色法) 96 次 PDF A-P-1036-96  
DNA甲基化定量试剂盒(荧光法) 48 次 PDF A-P-1035-48 点击这里给我发消息
DNA甲基化定量试剂盒(荧光法) 96 次 PDF A-P-1035-96  
DNA甲基化定量试剂盒(比色法) 48 次 PDF A-P-1034-48 点击这里给我发消息
DNA甲基化定量试剂盒(比色法) 96 次 PDF A-P-1034-96  
通用DNA甲基化定量试剂盒 48 次 PDF A-P-1011-48 点击这里给我发消息
通用DNA甲基化定量试剂盒 96 次 PDF A-P-1011-96  
超敏DNA甲基化定量试剂盒 48 次 PDF A-P-1021-48 点击这里给我发消息
超敏DNA甲基化定量试剂盒 96 次 PDF A-P-1021-96  
总DNA甲基化定量试剂盒--热销中 48 次 PDF A-P-1014B-48 点击这里给我发消息
总DNA甲基化定量试剂盒--热销中 96 次 PDF A-P-1014B-96  

推荐阅读:

关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPI研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法),货号是:A-P-1037/A-P-1036。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。

 
 
网站导航 | AAT Bioquest | IVDSHOW | Abnova | Active Motir | BioVision | Cayman | Caisson | Cyanagen | Equitech-Bio | Epigentek | Jackson | GeneMarkBio | Trevigen | ProSpec | ZYMO | [More>...]

 
 

版权所有:艾德科技(北京)有限公司  Tel:+86-0313-5935520 /0313-5935521 ADD:北京市昌平区中关村生命科学园东60米艾德园1951545998@qq.com P.C.:102206 网站管理 企业邮箱 微博 微信 易企秀 视频号 BiliBili-IVDSHOW 抖音

战略合作单位:【喀斯玛】【动物所】【清华】【北大】【北医】【农科物资】【供应室】【军科院】【WLT】【RJ2.0】【首医】【厦大】【广中大】【东大】【高校竞价】                                    

微信号:AD-Bio 订阅号:Bio-888 工程师:hugasis 工程师:JC57225208 工程师:15968826961 视频号:艾维缔 BiliBili实验视频

 

备案号:ICP11015760号-1  客服1:点击这里给我发消息  客服2:点击这里给我发消息 客服3:点击这里给我发消息 

 免责申明:本网站销售的所有产品均不得用于人类或动物之临床诊断或治疗,仅可用于工业或者科研等非医疗目的。(获得国家相关部门批准的产品除外)

22.1K