甲酰基(5-fC)定量检测试剂盒(荧光法) 货号：A-P-1042
MethylFlash 5-Formylcytosine (5-fC) DNA Quantification Kit (Fluorometric)
产品背景：The MethylFlash™ 5-Formylcytosine (5-fC) DNA Quantification Kit (Fluorometric) is a complete set of optimized buffers and reagents to colorimetrically quantify 5-formylcytosine (5-fC) in a microplate-based format. It is suitable for use with DNA isolated from any species (mammals, plants, fungi, bacteria, viruses, etc.) in a variety of forms including, but not limited to, cultured cells, fresh and frozen tissues, paraffin-embedded tissues, and body fluid samples.
Background Information
5-formylcytosine (5-fC) has recently been found in mammalian tissue and cells and has been classified as a seventh DNA base. It is formed by the oxidation of 5-hydroxymethylcytosine through TET hydroxylases and has been demonstrated to be an intermediate of the DNA demethylation process. The function of 5-fC in gene regulation is not yet entirely clear, but it has been shown that 5-fC exhibits replication-dependent dilution during mouse pre-implantation development and could be functional in regulating pre-implantation development as a whole. The detection of 5-fC in various tissues and cells is important because 5-fC could be a marker to indicate active DNA demethylation. 5-fC can also be directly excised by Thymine DNA glycosylase (TDG) to allow subsequent base excision repair (BER) processing which converts modified cytosine back to its unmodified state.
The kit has the following advantages and features:
Fast and easy procedure completed within just 3 hours and 45 minutes.
High sensitivity, of which the detection limit can be as low as 1 pg of 5-fC. High specificity with detection of only 5-fC.
No cross-reactivity to cytosine, 5-mC, 5-hmC, or 5-caC within the indicated concentration range of the sample DNA.
Highly convenient assay that uses DNA isolated from cells or tissues directly; no need for DNA digestion or hydrolysis.
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| 甲酰基(5-fC)定量检测试剂盒(荧光法) |
48 次 |
PDF |
A-P-1042-48 |
 |
| 甲酰基(5-fC)定量检测试剂盒(荧光法) |
96 次 |
PDF |
A-P-1042-96 |
|
表观遗传学产品全面解决方案列下：
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关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPI研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)，货号是:A-P-1037/A-P-1036。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
