甲酰基(5-fC)定量检测试剂盒(比色法) 货号：A-P-1041
MethylFlash 5-Formylcytosine (5-fC) DNA Quantification Kit (Colorimetric)
产品背景：The MethylFlash™ 5-Formylcytosine (5-fC) DNA Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to colorimetrically quantify 5-formylcytosine (5-fC) in a microplate-based format. It is suitable for use with DNA isolated from any species (mammals, plants, fungi, bacteria, viruses, etc.) in a variety of forms including, but not limited to, cultured cells, fresh and frozen tissues, paraffin-embedded tissues, and body fluid samples.
The kit has the following advantages and features:
Fast and easy procedure completed within just 3 hours and 45 minutes.
High sensitivity, of which the detection limit can be as low as 1 pg of 5-fC. High specificity with detection of only 5-fC.
No cross-reactivity to cytosine, 5-mC, 5-hmC, or 5-caC within the indicated concentration range of the sample DNA.
Highly convenient assay that uses DNA isolated from cells or tissues directly; no need for DNA digestion or hydrolysis.
Background Information
5-formylcytosine (5-fC) has recently been found in mammalian tissue and cells and has been classified as a seventh DNA base. It is formed by the oxidation of 5-hydroxymethylcytosine through TET hydroxylases and has been demonstrated to be an intermediate of the DNA demethylation process. The function of 5-fC in gene regulation is not yet entirely clear, but it has been shown that 5-fC exhibits replication-dependent dilution during mouse pre-implantation development and could be functional in regulating pre-implantation development as a whole. The detection of 5-fC in various tissues and cells is important because 5-fC could be a marker to indicate active DNA demethylation. 5-fC can also be directly excised by Thymine DNA glycosylase (TDG) to allow subsequent base excision repair (BER) processing which converts modified cytosine back to its unmodified state.
实验图片：
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| 说明 |
以不同浓度的5fC对照加入到孔中，然后采用比色法定量检测 5fC的含量。 |
通过甲酰基(5-fC)定量检测试剂盒(比色法)特异性检测5-fC的含量。将合成的未甲基化DNA、甲基化DNA、羟甲基化DNA和甲酰基DNA以不同浓度加入到孔中，使用甲酰基(5-fC)定量检测试剂盒(比色法)来进行检测所得数据。 |
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| 甲酰基(5-fC)定量检测试剂盒(比色法) |
48 次 |
英文PDF-中文PDF |
A-P-1041-48 |
 |
| 甲酰基(5-fC)定量检测试剂盒(比色法) |
96 次 |
英文PDF-中文PDF |
A-P-1041-96 |
|
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
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| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
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A-P-1037-48 |
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| DNA甲基化定量试剂盒(荧光法) |
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PDF |
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| 超敏DNA甲基化定量试剂盒 |
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关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPI研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)，货号是:A-P-1037/A-P-1036。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
