重组 p53 蛋白，带活性的 货号：E-31465
Recombinant p53 protein
背景资料：p53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, an ubiquitin ligase specific for p53, which causes its destabilization. Inactivation of p53 is often coincident with hyperactivation of NFκB (NFκB p50 andNFκB p65), both of which serve to inhibit apoptosis.
产品描述：
p53 is a tumor suppressor protein functioning as a sequence-specific transcription factor important for cell cycle arrest, apoptosis, and DNA-damage response. Recombinant p53, transcript variant 1, was expressed in E. coli from human cDNA (accession number NM_000546.5) with an N-terminal polyhistidine tag.
Active Motif also offers Recombinant p53 protein (Catalog No. 31103) that was expressed in Baculovirus.
产品应用：
Recombinant p53 is suitable for use in Active Motif’s TransAM® assay as a protein standard, in vitro transcription assays, protein-protein interactions and protein-DNA interaction studies. A recommended starting point for in vitro transcription assays is 20-100 ng per reaction. The molecular weight of the protein is 53 kDa.
产品使用：
10 µg recombinant p53 protein supplied at a concentration of 0.38 µg/µl in 50 mM Tris-HCl, pH 8, 200 mM NaCl, 5 mM beta-mercaptoethanol and 5% glycerol.This protein is described in the following reference:
Horton J et al. (2010) Nature 17:38-43
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
表观遗传学产品全面解决方案列下：
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