重组 核小体 (H3.1) 货号：E-31466
Recombinant Nucleosomes (H3.1)
背景资料：n vivo, histones are wrapped around by DNA in chromatin. Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides forin vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery.
产品描述：
Recombinant Nucleosomes (H3.1), Human consists of 5000 bp of DNA (plasmid pG5E4) and two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NM_003512), H2B that includes amino acids 1-126 (end) (accession number NM_003518), H3.1 that includes amino acids 1-136 (end) (accession number NM_003529), and H4 that includes amino acids 1-103 (end) (accession number NM_003548). Plasmid pG5E4 includes 11 5S rDNA nucleosome positioning sequences of L. variegatus. Every 5S rDNA can wrap one histone octamer to form a nucleosome. Recombinant nucleosome (H3.1) is an oligonucleosome including at least 11 nucleosomes. Recombinant Nucleosomes (H3.1), Human was generated in E. coli cells and has an observed molecular weight of 108 kDa. The recombinant protein is >95% pure by SDS-PAGE.
产品应用：
Recombinant Nucleosomes (H3.1) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
HMT Assay Conditions: 50 mM TrisCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM, 30 ng/μl Recombinant Nucleosomes (H3.1), 30 ng/μl DOT1L (1-416 aa) at 2 hours at room temperature. Activity was detected by fluorography.
产品使用：
Recombinant Nucleosomes (H3.1) (20 µg protein + 24 µg DNA) is supplied at a concentration of 0.55 µg/µl in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 2 mM DTT and 20% glycerol.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
表观遗传学产品全面解决方案列下：
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