LightSwitch™ 启动子 GoClone™ 货号：E-32001
LightSwitch™ Promoter Reporter GoClone™
背景资料：The LightSwitch™ Luciferase Assay System includes over 30,000 regulatory elements from the human genome that have been cloned into LightSwitch reporter vectors. With pre-cloned human promoters, lncRNA promoters and 3´UTRs available as transfection-ready LightSwitch luciferase reporter constructs, you can perform your reporter assay experiments immediately. Especially convenient are the Validated Pathway Collections, which are promoter constructs that showed significant activation or repression in response to in-house, pathway-specific induction testing. The LightSwitch System makes it easy for you to measure the transcriptional and post-transcription regulation of any gene in the genome.
To ensure high-quality results, LightSwitch reporter vectors utilize a novel, engineered luciferase gene (RenSP) and optimized reagents that provide industry-leading sensitivity. For more information, please see the tabs below as well as the Overviewand RenSP Luciferase sections on the LightSwitch Luciferase Assay System page.
产品描述：
The LightSwitch Promoter Reporter Collection makes it possible to measure the functional response of promoters to stimulus in nearly any cell-based system. Plus, advances in gene assay reagent and transfection technology eliminate the need for co-transfection in most studies.
产品应用：
Studying transcription factor activity with the LightSwitch System
Transcription factors are important regulators of eukaryotic transcriptional regulation, and they interact with DNA-encoded regulatory elements like promoters to modulate when, where and how much of a gene’s mRNA gets made. The activity of transcription factors can be affected by the cell’s environmental conditions including exposure to signaling molecules like hormones and growth factors. Reporter assays are a powerful technique for measuring the activity of promoters in living cells. LightSwitch Reporter Constructs are created by isolating a promoter from the human genome and cloning it upstream of the RenSP luciferase reporter gene on a plasmid. Once this plasmid is transfected into a living cell, a change in promoter activity causes a change in the reporter signal (light output).
产品使用：
Find and order LightSwitch™ GoClones™
To find LightSwitch GoClone constructs that contain the promoters of your genes of interest, use the box below to search the LightSwitch Promoter Reporter Collection. Place your order using the specific Product ID(s) you find for the clone(s) you wish to order. As we cannot stock every GoClone in all of our offices, delivery may take 10-14 days.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1036-48 |
 |
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1036-96 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1037-48 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1037-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1034-96 |
|
| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
英文PDF--中文PDF |
A-P-1014B-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
英文PDF--中文PDF |
A-P-1014B-96 |
|
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关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
