LightSwitch™ LDHA 启动子 对照 货号:E-32005
LightSwitch™ LDHA Promoter Control
背景资料:Because no single promoter can be an ideal control for all experimental conditions, Active Motif offers a panel of positive and negative control constructs for the LightSwitch™ Promoter Reporter Vector. These controls are used to measure background, to optimize experimental conditions and to normalize for non-specific effects associated with a treatment or change in condition. Note that the unique RenSP luciferase technology utilized in the LightSwitch Assay System eliminates the need for co-transfection of a normalizing control in most cases. So, instead of one control construct being co-transfected with every experimental plasmid, different control vectors can be used in your reporter assay experiment, each in its own well, in addition to the constructs being tested. Using the most suitable positive and negative control vectors for your specific experiment will give you the highest degree of confidence in your results.
产品描述:
MPORTANT: Because all LightSwitch reporter constructs contain the optimized RenSP luciferase gene, you MUST use our LightSwitch Luciferase Assay Reagents to obtain optimal results. (Other luciferase assay reagents are not compatible with RenSP.)
产品应用:
Empty promoter vector: The empty promoter vector contains the RenSP luciferase reporter gene without a promoter. This construct serves as a measure of background signal in the experiment. This construct may also be used to create additional experimental constructs. For a vector map, annotations and primer & restriction sites, please see the Empty LightSwitch™ Reporter Vectors page.
Housekeeping gene promoter vectors: Housekeeping control constructs contain promoters for common housekeeping genes driving the RenSP luciferase reporter gene. These constructs serve as positive transfection controls and may also serve as controls for comparing signals between conditions if they are known to be unresponsive to the test condition.
Random control promoter vectors: Random control constructs contain 1 kb non-conserved, non-genic, non-repetitive fragments from the human genome cloned upstream of the RenSP luciferase reporter gene. These vectors produce slightly different signals than the empty promoter vector and are considered optimal negative or background controls.
产品使用:
Information on all LightSwitch Promoter Control Vectors is shown under the Promoter Controls tab below.
温度保存:
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息:
表观遗传学产品全面解决方案列下:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
DNA羟甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1036-48 |
 |
DNA羟甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1036-96 |
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DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1037-48 |
 |
DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1037-96 |
|
DNA甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1035-48 |
 |
DNA甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1035-96 |
|
DNA甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1034-48 |
 |
DNA甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1034-96 |
|
通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
 |
通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
 |
超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
总DNA甲基化定量试剂盒 |
48 次 |
英文PDF--中文PDF |
A-P-1014B-48 |
 |
总DNA甲基化定量试剂盒 |
96 次 |
英文PDF--中文PDF |
A-P-1014B-96 |
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