重组 SIRT1 蛋白,带活性的 货号:E-31340
Recombinant SIRT1 protein
背景资料:SIRT1 (Sirtuin 1, SIR2L1) is a member of the Sirtuin family of proteins related to the yeast Sir2 protein. Like Sir2 and other Sirtuins, SIRT1 is an NAD-dependent histone and protein deacetylase. SIRT1 catalyzes the removal of acetyl groups from a variety of protein substrates (NBS1, p53, Ku70, etc.) as well as from Histone H4 Lys16 (H4K16).SIRT1 is involved in the regulation of a number of cellular processes, including transcription, metabolism, DNA repair and aging, and increased SIRT1 is associated with extended lifespan of C. elegans.
产品描述:
SIRT1 is a histone and protein deacetylase, an NAD-dependant enzyme that removes acetyl groups from lysine acetylated proteins. SIRT1 has been shown to deacetylate histone H4 at lysine 16. Recombinant human SIRT1 was expressed in E. coli as the full length protein (accession number AAD40849) with an N-terminal FLAG tag. The molecular weight of the protein is ~120 kDa.
产品应用:
Recombinant SIRT1 is suitable for use in histone deacetylase assays. A recommended starting point for the HDAC assay is 25-100 ng of recombinant protein per reaction.
产品使用:
Recombinant SIRT1 protein supplied at a concentration of 0.2 µg/µl in 20 mM Tris-HCl (pH7.3), 300 mM KCl, 0.2 mM EDTA and 20% glycerol.
温度保存:
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息:
表观遗传学产品全面解决方案列下:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
DNA羟甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1036-48 |
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DNA羟甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1036-96 |
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48 次 |
英文PDF--中文PDF |
A-P-1037-48 |
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96 次 |
英文PDF--中文PDF |
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48 次 |
英文PDF--中文PDF |
A-P-1035-48 |
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DNA甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1035-96 |
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DNA甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1034-48 |
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96 次 |
英文PDF--中文PDF |
A-P-1034-96 |
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48 次 |
PDF |
A-P-1011-48 |
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通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
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超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
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96 次 |
PDF |
A-P-1021-96 |
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总DNA甲基化定量试剂盒 |
48 次 |
英文PDF--中文PDF |
A-P-1014B-48 |
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96 次 |
英文PDF--中文PDF |
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