重组 HDAC1 蛋白，带活性的 货号：E-31342
Recombinant HDAC1 protein
背景资料：HDAC1 (Histone Deacetylase 1, also designated HD1) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.
In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7,HDAC9 and 10), class III (SIRT1-7) and class IV (HDAC11).
HDAC1 and HDAC2 are recruited to Mad-Max complexes, which associate with the Sin3 scaffold protein, and are required for the transcriptional repression of Mad-Max target genes. HDAC1 is also involved in the regulation of p53. HDAC1 is expressed in various tissues. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate.
产品描述：
HDAC1 is a histone deacetylase with broad specificity. Recombinant human HDAC1 was expressed in a baculovirus expression system as the full length protein (accession number NM_004964) with a C-terminal His tag and C-terminal FLAG tag. The molecular weight of the protein is 56 kDa. This recombinant protein is ~69% pure by SDS-PAGE.
产品应用：
Recombinant HDAC1 is suitable for use in histone deacetylase assays. Specific Activity: 460 pmol/min/µg. This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay conditions: Prepare 25 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA, 20 μM HDAC substrate and recombinant HDAC1 protein (titrated at various amounts). Incubate for 30 minutes at 37°C followed by developing for 15 minutes at room temperature.
产品使用：
Full length recombinant HDAC1 protein supplied at a concentration of 0.1 µg/µl in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 100 ng/µl FLAG peptide and 20% glycerol.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
表观遗传学产品全面解决方案列下：
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