重组 TRIM33 (959-1069) 蛋白 货号：E-31367
Recombinant TRIM33 (959-1069) protein
背景资料：Tripartite motif-containing 33 (TRIM33) protein, also known as TIF1γ, is a member of the transcriptional intermediary factor 1 (TIF1) family that control transcription and chromatin remodeling through their interaction with transcription factors. The family includes TRIM24 (TIF1α), TRIM28 (TIF1β) and TRIM33 (TIF1γ) that share a characteristic domain structure comprised of multiple histone-binding domains, an N-terminal TRIM region (containing a RING domain, B box type 1 and type 2 domains, and a coiled-coil region), and a C-terminal bromodomain and PHD finger. Bromodomainsfunction as ‘readers’ of epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TRIM33 is an E3 ubiquitin protein ligase that promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. TRIM33 does not affect SMAD4 levels but rather acts as an inhibitor of the SMAD4-dependent TGFβ/BMP signaling cascade by monoubiquitinating SMAD4 and hampering its ability to form a stable complex with activated SMAD2/3. TRIM33 may also act as a transcriptional corepressor. It has also been shown to associate with SMAD2 and SMAD3 and stimulate differentiation of hematopoietic stem cells. Additionally, TRIM33 associates with TRIM24 and plays a role in the control of cell proliferation.
产品描述：
The peptide corresponding to amino acids 959 - 1059 that contains the bromodomain sequences of TRIM33 (accession number NP_056990.3) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 17.7 kDa. The recombinant protein is >75% pure by SDS-PAGE.
产品应用：
Recombinant TRIM33 (959-1069) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
产品使用：
Recombinant TRIM33 (959-1069) protein expressed in E. coli at a concentration of 0.5 mg/ml in 25 mM HEPES pH 7.5, 150 mM NaCl and 5% glycerol.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
表观遗传学产品全面解决方案列下：
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