重组 G9a-SET 蛋白 货号：E-31425
Recombinant G9a-SET protein
背景资料:
G9a, also known as Euchromatic Histone-lysine N-methyltransferase 2 (EHMT2), is a histone methyltransferase that specifically mono- and dimethylates Lys9 of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by initiating recruitment of HP1 proteins to methylated histones. G9a also mediates monomethylation of Lys56 of histone H3 (H3K56me1) in G1 phase and regulates DNA replication by promoting the interaction between histone H3 and PCNA. G9a also weakly methylates Lys27 of histone H3 (H3K27me). In addition, G9a is required for DNA methylation. However, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. G9a is most likely targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. G9a may also methylate histone H1. In addition to histone methyltransferase activity, G9a also methylates non-histone proteins. It has been shown to mediate dimethylation of Lys373 of p53 and also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.
产品描述：
The peptide corresponding to amino acids 913 - 1193 of the G9a protein (accession number NP_006700.3) was expressed in E. coli. The peptide contains the SET domain of G9a that is responsible for methyltransferase activity. Recombinant G9a-SET contains N-terminal GST tag with an observed molecular weight of 58.7 kDa.
产品应用：
Recombinant G9a-SET is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
产品使用：
20 µg of recombinant G9a-SET protein expressed in E. coli at a concentration of 0.8 mg/ml in 20 mM Tris-HCl pH 7.4, 150 mM NaCl and 20% glycerol.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
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