重组 JARID1B / KDM5B 蛋白 货号：E-31832
Recombinant JARID1B / KDM5B protein
背景资料:
Lysine (K)-specific demethylase 5B (KDM5B), also known as Jumonji, AT rich interactive domain 1B (JARID1B), is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5B demethylates trimethylated, dimethylated and monomethylated H3K4. It does not demethylate histone H3K9 or H3K27. KDM5B functions as a transcriptional corepressor for FOXG1B and PAX9. KDM5B induces the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, it may act as a tumor suppressor for melanoma.
产品描述：
Recombinant JARID1B / KDM5B (accession number NP_006609, full length) was expressed in Sf9 and contains an N-terminal FLAG tag with a molecular weight of 179.9 kDa. The recombinant protein is >75% pure by SDS-PAGE.
产品应用：
Recombinant JARID1B / KDM5B is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Specific Activity: H3K4me3 demethylase.
Catalytic Rate: 20 turnovers/ enzyme molecule
Histone Demethylase Assay Conditions: 3.3 μM H3K4me3 (aa 1-21) peptide was incubated with different concentrations of recombinant JARID1B / KDM5B protein in reaction buffer containing 50 mM HEPES pH 7.5, 0.02% Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2·6H2O, 1 mM TCEP for 1 hours at room temperature. HTRF and MALDI-TOF were used for detection.
产品使用：
Full length recombinant JARID1B / KDM5B protein expressed in Sf9 at a concentration of 0.2 mg/ml in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP and 3X FLAG peptide.
This protein is described in the following references:
Yamane K et al. (2007) Mol Cell 25:801-812
Barrett A et al. (2007) Int J Cancer 121:265-275
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
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