细胞基因组DNA 货号：E-55007
Jurkat genomic DNA
背景资料：Active Motif's MethylCollector™ Ultra offers a fast magnetic assay capable of efficiently isolating methylated CpG islands from fragmented genomic DNA*. MethylCollector Ultra is based on the Methylated CpG Island Recovery Assay (MIRA) which uses a MBD2b/MBD3L1 protein complex for improved enrichment of CpG dinucleotides. Enriched DNA can be used in many downstream applications, such as end point or real time PCR analysis of the methylation status of particular loci in normal and diseased samples, bisulfite conversion followed by cloning and sequencing, or amplification and labeling for microarray analysis.
产品描述：MethylCollector™ Ultra is based on the Methylated CpG Island Recovery Assay (MIRA) which utilize a His-tagged recombinant methyl-binding protein complex, comprised of MBD2b/MBD3L1, that specifically binds methylated CpGs of genomic DNA fragments that have been prepared by sonication or enzymatic digestion. Nickel-coated magnetic beads capture the protein-DNA complexes which are separated from the rest of the genomic DNA using the included magnet. Optimized buffers ensure that fragments with little or no methylation are removed. The methylated DNA is then eluted from the beads in the presence of Proteinase K. Following clean up, the eluted DNA is ready for use in PCR analysis or other downstream applications. See Flow Chart of MethylCollector™ Ultra process below..
The Methylated CpG Island Recovery Assay (MIRA) utilizes the high affinity of the MBD2b/MBD3L1 complex for methylated DNA1, to provide better enrichment than assays that use the methyl-binding protein MBD2 alone2. MethylCollector™ Ultra can efficiently enrich for methylated DNA from as few as 170 cells (~1 ng DNA)
使用说明：Specificity of the MethylCollector™ Ultra Kit was also determined by comparing both the unbound and eluted fractions under both high and low salt conditions (Figures 2 & 3). Quality control testing was performed on 100 ng of Mse I digested human, male genomic DNA included in the kit. Enrichment of CpG-methylated DNA was confirmed using real-time PCR with the provided NBR2 (methylated promoter), Xist (methylated promoter) and GAPDH (unmethylated promoter) PCR primer mixes. The methylated NBR2 and Xist promoters gave robust signals and showed strong enrichment in the eluted fraction as compare to the unbound fraction. The unmethylated GAPDH promoter was only detected in the unbound fractions. These results verify the specificity of the MBD2b/MBD3L1 protein complex for methylated DNA. The level of enrichment may vary depending on the source of the DNA sample and the locus being evaluated.
产品应用：The ability of MethylCollector™ to specifically enrich for methylated DNA over alternative methods such as MBD-Biotin capture, or antibody-based immunoprecipitation is shown in Figure 1. A direct comparison of eluted DNA using PCR primers for both unmethylated and methylated promoters reveals the ability of MethylCollector™ Ultra to specifically isolate CpG-methylated DNA. DNA eluted from the other methods showed little to no enrichment as both unmethylated and methylated promoters amplified at approximately the same number of PCR cycles.
保存建议：His-MBD2b/MBD3L1 protein complex, High Salt Binding Buffer, Low Salt Binding Buffer, Elution Buffer AM1, Protease Inhibitors, Proteinase K, Proteinase K Stop Solution, Magnetic Nickel Beads, Mse I digested Human, male genomic DNA, NBR2 PCR Primer Mix methylated control, Xist PCR Primer Mix methylated control, GAPDH PCR Primer Mix unmethylated control, Glycogen, PCR Buffer, Loading Dye, Bar Magnet, Glue dots and PCR tubes. Storage conditions vary from room temperature to -20°C, please see manual for specific details. All products guaranteed for 6 months from date of receipt.
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| 细胞基因组DNA |
10ug |
PDF |
E-55007 |
 |
| 重组 DNMT1 蛋白, active |
10ug |
PDF |
E-31335 |
|
| 6-Methyladenosine (6-meA) 抗体 (pAb) |
100ug |
PDF |
E-61495 |
|
| 6-Methyladenosine (6-meA) 抗体 (pAb) |
10ug |
PDF |
E-61496 |
|
| DNMT3A dimethyl Lys44 抗体 (pAb) |
100ul |
PDF |
E-61323 |
|
| DNMT3A dimethyl Lys44 抗体 (pAb) |
10ul |
PDF |
E-61324 |
|
| DNMT3A 抗体 (mAb) |
200ug |
PDF |
E-39206-200 |
|
| DNMT3A 抗体 (mAb) |
100ug |
PDF |
E-39206-100 |
|
| 3-Methylcytosine (3-mC) 抗体 |
100ug |
PDF |
E-61111 |
|
| 3-Methylcytosine (3-mC) 抗体 |
10ug |
PDF |
E-61112 |
|
| CRISPR/Cas9 单克隆抗体【7A9】 |
100ug |
PDF |
E-A-9000-100 |
| CRISPR/Cas9 单克隆抗体【7A9】 |
50 ug |
PDF |
E-A-9000-50 |
|
| CRISPR/Cas9 单克隆抗体【7A9】 |
10 ug |
PDF |
E-A-9000-10 |
|
| CRISPR/Cas9 多克隆抗体 |
100ug |
PDF |
E-A-1111-100
|
|
| CRISPR/Cas9 多克隆抗体 |
50 ug |
PDF |
E-A-1111-050
|
|
| CRISPR/Cas9 多克隆抗体 |
10 ug |
PDF |
E-A-1111-010
|
|
| APC标记大鼠抗小鼠CD90.2/Thy-1.2抗体 |
0.1mg |
PDF |
E-1750-11 |
|
| PE标记仓鼠抗小鼠CD69/VEA抗体 |
0.2mg |
PDF |
E-1715-09L |
|
| 低内毒素/无叠氮钠仓鼠抗小鼠CD3e抗体 |
0.5mg |
PDF |
E-1531-14 |
|
| 生物素标记山羊抗I型胶原蛋白抗体 |
0.2mg |
PDF |
E-1310-08 |
|
| 兔抗人DR5抗体 |
0.1mg |
PDF |
E-6600-01 |
|
| SEPH标记山羊抗s-tag标签抗体 |
1.0ml |
PDF |
E-6500-25 |
|
| 抗小鼠TREM-2单克隆抗体(克隆号6E9) |
100ug |
PDF |
C-HM1129F |
|
| 96孔DNA甲基化极速修饰试剂盒（磁珠法） |
192次 |
英文PDF--中文PDF |
A-P-1050-192 |
|
| m6A RNA甲基化定量检测试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-9005-96 |
|
| RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-P-9003-050 |
|
| EZ RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-R5001 |
|
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1036-48 |
|
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1036-96 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1037-48 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1037-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1034-96 |
|
| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
英文PDF--中文PDF |
A-P-1014B-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
英文PDF--中文PDF |
A-P-1014B-96 |
|
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GAPDH, glyceraldehyde-3-phosphate dehydrogenase, is important for metabolism. Because this gene is often constitutively expressed, it is considered to be an actively transcribed housekeeping gene containing an unmethylated promoter in healthy tissues. The region amplified by this primer pair is 69 base pairs and contains 7 CpGs.
Xist, X inactive specific transcript, is a methylated promoter in human, male genomic DNA, but is non-methylated in females. The region amplified by this primer pair is 178 base pairs and contains 8 CpGs.
NBR2, neighbor of BRCA1 gene 2 is located near the breast cancer gene BRCA1. Evidence indicates that NBR2 and BRCA1 share a bi-directional promoter. This region of the NBR2 gene is methylated promoter in healthy tissues. The region amplified by this primer pair is 103 base pairs and contains 7 CpGs |
