完整甲基化细胞DNA 货号：E-55003
Fully Methylated Jurkat DNA
背景资料：6-Methyladenosine (6-meA) is an RNA modification on the N-6 position of adenosine. This modification has been found to be abundant in the 3’ UTR and stop codons of mammalian mRNA. 6meA is associated with miRNA binding sites suggesting a potential role in epigenetic gene regulation. FTO and ALKBH are demethylases for 6-methyladenosine while a multitprotein complex that includes METTL3 functions as the methyltransferase参考文献：1. Rauch, T. and Pfeifer, G. (2005) Lab. Investigations, 85: 1172-1180.
MethylCollector™ Ultra is based on the Methylated CpG Island Recovery Assay (MIRA) which utilize a His-tagged recombinant methyl-binding protein complex, comprised of MBD2b/MBD3L1, that specifically binds methylated CpGs of genomic DNA fragments that have been prepared by sonication or enzymatic digestion. Nickel-coated magnetic beads capture the protein-DNA complexes which are separated from the rest of the genomic DNA using the included magnet. Optimized buffers ensure that fragments with little or no methylation are removed. The methylated DNA is then eluted from the beads in the presence of Proteinase K. Following clean up, the eluted DNA is ready for use in PCR analysis or other downstream applications. See Flow Chart of MethylCollector™ Ultra process below.
The Methylated CpG Island Recovery Assay (MIRA) utilizes the high affinity of the MBD2b/MBD3L1 complex for methylated DNA1, to provide better enrichment than assays that use the methyl-binding protein MBD2 alone2. MethylCollector™ Ultra can efficiently enrich for methylated DNA from as few as 170 cells (~1 ng DNA).
2.Jiang, C.L. et al. (2004) J. Bio. Chem., 279: 52456-52464。
使用说明：Active Motif's MethylCollector™ Ultra offers a fast magnetic assay capable of efficiently isolating methylated CpG islands from fragmented genomic DNA*. MethylCollector Ultra is based on the Methylated CpG Island Recovery Assay (MIRA) which uses a MBD2b/MBD3L1 protein complex for improved enrichment of CpG dinucleotides. Enriched DNA can be used in many downstream applications, such as end point or real time PCR analysis of the methylation status of particular loci in normal and diseased samples, bisulfite conversion followed by cloning and sequencing, or amplification and labeling for microarray analysis.
保存建议：Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storageHis-MBD2b/MBD3L1 protein complex, High Salt Binding Buffer, Low Salt Binding Buffer, Elution Buffer AM1, Protease Inhibitors, Proteinase K, Proteinase K Stop Solution, Magnetic Nickel Beads, Mse I digested Human, male genomic DNA, NBR2 PCR Primer Mix methylated control, Xist PCR Primer Mix methylated control, GAPDH PCR Primer Mix unmethylated control, Glycogen, PCR Buffer, Loading Dye, Bar Magnet, Glue dots and PCR tubes. Storage conditions vary from room temperature to -20°C, please see manual for specific details. All products guaranteed for 6 months from date of receipt.
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| 完整甲基化细胞DNA |
10ug |
PDF |
E-55003 |
 |
| 重组 DNMT1 蛋白, active |
10ug |
PDF |
E-31335 |
|
| 6-Methyladenosine (6-meA) 抗体 (pAb) |
100ug |
PDF |
E-61495 |
|
| 6-Methyladenosine (6-meA) 抗体 (pAb) |
10ug |
PDF |
E-61496 |
|
| DNMT3A dimethyl Lys44 抗体 (pAb) |
100ul |
PDF |
E-61323 |
|
| DNMT3A dimethyl Lys44 抗体 (pAb) |
10ul |
PDF |
E-61324 |
|
| DNMT3A 抗体 (mAb) |
200ug |
PDF |
E-39206-200 |
|
| DNMT3A 抗体 (mAb) |
100ug |
PDF |
E-39206-100 |
|
| 3-Methylcytosine (3-mC) 抗体 |
100ug |
PDF |
E-61111 |
|
| 3-Methylcytosine (3-mC) 抗体 |
10ug |
PDF |
E-61112 |
|
| CRISPR/Cas9 单克隆抗体【7A9】 |
100ug |
PDF |
E-A-9000-100 |
| CRISPR/Cas9 单克隆抗体【7A9】 |
50 ug |
PDF |
E-A-9000-50 |
|
| CRISPR/Cas9 单克隆抗体【7A9】 |
10 ug |
PDF |
E-A-9000-10 |
|
| CRISPR/Cas9 多克隆抗体 |
100ug |
PDF |
E-A-1111-100
|
|
| CRISPR/Cas9 多克隆抗体 |
50 ug |
PDF |
E-A-1111-050
|
|
| CRISPR/Cas9 多克隆抗体 |
10 ug |
PDF |
E-A-1111-010
|
|
| APC标记大鼠抗小鼠CD90.2/Thy-1.2抗体 |
0.1mg |
PDF |
E-1750-11 |
|
| PE标记仓鼠抗小鼠CD69/VEA抗体 |
0.2mg |
PDF |
E-1715-09L |
|
| 低内毒素/无叠氮钠仓鼠抗小鼠CD3e抗体 |
0.5mg |
PDF |
E-1531-14 |
|
| 生物素标记山羊抗I型胶原蛋白抗体 |
0.2mg |
PDF |
E-1310-08 |
|
| 兔抗人DR5抗体 |
0.1mg |
PDF |
E-6600-01 |
|
| SEPH标记山羊抗s-tag标签抗体 |
1.0ml |
PDF |
E-6500-25 |
|
| 抗小鼠TREM-2单克隆抗体(克隆号6E9) |
100ug |
PDF |
C-HM1129F |
|
| 96孔DNA甲基化极速修饰试剂盒（磁珠法） |
192次 |
英文PDF--中文PDF |
A-P-1050-192 |
|
| m6A RNA甲基化定量检测试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-9005-96 |
|
| RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-P-9003-050 |
|
| EZ RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-R5001 |
|
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1036-48 |
|
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1036-96 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1037-48 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1037-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1034-96 |
|
| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
英文PDF--中文PDF |
A-P-1014B-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
英文PDF--中文PDF |
A-P-1014B-96 |
|
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关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
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时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
