重组 Tet1 蛋白，带活性的 货号：E-31363
Recombinant Tet1 protein, active
背景资料：Ten-eleven Translocation Gene Protein 1 (Tet1) is a DNA-binding protein that modulates DNA methylation and gene transcription via hydroxylation of 5-methylcytosine (5-mC). Tet1 is a cytosine oxygenase that catalyzes the conversion of the modified genomic base 5-mC into 5-hydroxymethylcytosine (5-hmC). Methylation at the C5 position of cytosine is an epigenetic modification of the mammalian genome that plays an important role in transcriptional regulation. Tet1 is responsible for initiating the enzymatic deamination process leading to cytosine demethylation. Tet1 preferentially binds to CpG-rich sequences at the promoters of both transcriptionally active and Polycomb-repressed genes and modulates gene silencing induced by cytosine methylation by controlling the levels of 5-mC and 5-hmC at gene promoters. Tet1 may have the dual function of repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters. Tet1 is important for stem cell maintenance and specification and is involved in the balance between pluripotency and lineage commitment.
产品组成：25 µg recombinant Tet1 protein expressed in E. coli supplied in a buffer of 20 mM Tris 8.0, 300 mM NaCl, 2 mM DTT and 5% glycerol.
蛋白来源：Mouse
内在表达：E. coli
产品详情：Tet1 is a member of the cytosine oxygenase family of enzymes that convert 5-methylcytosine to 5-hydroxymethylcytosine. Amino acids 1367 to 2039 of mouse Tet1 (accession number ACY38291.1) were expressed in E. coli (MW=95 kDa)
产品应用：Recombinant Tet1 protein is suitable for use in conversion of 5-methylcytosine containing DNA into DNA containing 5-hydroxymethylcytosine. A good starting point is 0.5 to 5 μg of enzyme per assay incubated with 0.5 μg of substrate in 50 mM HEPES, pH 6.8, 50 μM Fe(NH4)2(SO4)2, 2 mM ascorbate, and 1 mM alpha-ketoglutarate for 3 hr at 37°C.
保存建议：Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| 重组 Tet1 蛋白，带活性的 |
25ug |
PDF |
E-31363 |
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100ul |
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50 ug |
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|
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表观遗传学产品全面解决方案列下：
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5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
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