MASP-3(人)ELISA试剂盒                                 货号：C-HK339-02

MASP-3, Human, ELISA kit

背景资料：The MASP-3 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 4·5 hours· The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are captured by a solid bound specific antibody· Biotinylated tracer antibody will bind to captured MASP-3· Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody· Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of oxalic acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the MASP-3 standards (log)· The MASP-3 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve。

产品描述：The mannan-binding lectin (MBL) pathway of the complement system is initiated by the binding of MBL or ficolin to specific carbohydrate structures found on the surface of a variety of microorganisms· This binding is followed by the activation of MBL-associated serine proteases (MASPs)· There are four known MASPs: MASP-1, MASP-2, MASP-3 and Map19 (also known as sMAP, a small, separately synthesized fragment of MASP-2)· MASPs are secreted as single chain precursors and are processed into a heavy chain (A) and catalytic light chain (B) linked by disulfide bonds· All MASPs form homo-dimers and individually associate with MBL and the ficolins in a Ca2+-dependent manner· MASP-3 is an alternatively spliced product from the MASP-1 gene, containing an identical A chain, but an entirely different B chain· MASP-3 is an approximately 100 kDa protein and associates with both MBL and L-ficolin in the presence of Ca2+· The MASP3 dimer can be disrupted by EDTA· MASP-3 is expressed mainly but not exclusively in the liver· Liver expressed MASP-3 is secreted into the blood· Unlike MASP-2, no proteolytic activity on complement proteinss C2, C3 and C4 for MASP-3 has been detected· MASP-3 exerts inhibitory activity on MASP-2 function· However, no natural substrates for MASP-3 are known· MASP-3 concentration in healthy human serum is normally distributed with a mean value of 3796 ng/ml· MASP-3 is present at birth at approximately 80% of the level measured 6, 9 and 12 months after birth· The MASP-3 level is stable throughout a 1-year period· The high expression of MASP-3 in astrocytes indicates that MASP-3 might also be involved in other functions that could be important in the brain· In multiple sclerosis patients, high MASP-3 levels seem to have a positive effect (median MASP-3 levels: 4539 ng/ml)· MASP-3 deficiencies are currently not reported。

产品特点：he human MASP-3 ELISA kit is to be used for the in vitro quantitative determination of both natural and recombinant human MASP-3· This kit is intended for laboratory research use only and is not for use in diagnostic or therapeutic procedures· In serum or plasma samples MASP-3 can be measured accurately if serum or plasma samples are diluted at least 20 times· The cross reactivity with Human MASP-1 and Human MASP-2 was negative。

其他： 荷兰Hycult Biotech的宗旨和策略是着重于天然免疫相关疾病的研究，为人类生活质量的提高谋福利。作为免疫学和细胞生物学领域的领导者，特别是天然免疫、炎症反应和细胞损伤等相关领域的领先者，Hycult Biotech公司不仅研发这些用于生物科学研究的产品，同时也致力于将这些科研产品转化为临床测试的研究。作为多家知名品牌的中国总代理以及中国区域代理，艾德科技与Hycult一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量。

保存建议：推荐4℃保存，有效期一年。

定购信息： 

产品名称	规格	操作手册	货号	询价
MASP-3(人)ELISA试剂盒	1*96det.	PDF	C-HK339-01
MASP-3(人)ELISA试剂盒	2*96det.	PDF	C-HK339-02
【rIL 3 】重组大鼠白介素-3	5 ug	PDF	C-YT-383-1
【rIL 3 】重组大鼠白介素-3	20ug	PDF	C-YT-383-2
【rIL 3 】重组大鼠白介素-3	1 mg	PDF	C-YT-383-3

表观遗传学产品全面解决方案列下： 

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化试剂盒(定性分析)	48 次	PDF	A-P-1011-48
通用DNA甲基化试剂盒(定性分析)	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

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