弹力素(人)ELISA试剂盒                                  货号： C-HK318-02

Elafin, Human, ELISA kit

背景资料：The human elafin ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3·5 hours· The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are captured by a solid bound specific antibody· Biotinylated tracer antibody will bind to captured human elafin· Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody· Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of oxalic acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human elafin standards (log)· The human elafin concentration of samples, which are run concurrently with the standards, can be determined from the standard curve。

产品描述：Elafin is an epithelial proteinase inhibitor also known under various other names such as Skin-derived Anti leukoproteinase (SKALP) and Elastase-Specific Inhibitor (ESI)· Elafin belongs to the Trappin gene family and was given the systematic name Trappin-2 in a recent classification· The Trappin family is defined by a N-terminal transglutaminase substrate domain and a C-terminal four disulphide core· Trappins have been suggested to play a role in the regulation of inflammation and in protection against tissue damage in stratified epithelia· Elafin is an inhibitor of leukocyte elastase and proteinase-3 and in addition it is a substrate for transglutaminases· The protein is constitutively expressed in various epithelia including those of hair follicles, oesophagus, vagina and oral cavity· Elafin is not present in normal human skin but is strongly induced during inflammation as in psoriasis and wound healing· The full-length protein is translated as a 12·3 kDa protein of 117 amino acids termed pre-elafin or Trappin-2· Cleavage of the signal peptide yields a mature protein with a molecular mass of 9·9 kDa· The 9·9 kDa secreted protein is the major form found in culture medium· In skin extracts a 6 kDa form comprising the 57 most C-terminal amino acids is present, which is the form of elafin originally described by Wiedow et al· In serum both the 9·9 and the 6 kDa form appear to be present· In urine only the short 6 kDa form is found· The HYCULT BIOTECH ELISA is not suitable to measure the short 6 kDa form· Elafin has been used as a quantitative marker for disease activity in severe psoriasis during treatment with cyclosporin· In serum/plasma of healthy individuals approximately 10-50 ng/ml elafin is present· Elafin shows a 10-fold increase in concentration during psoriasis· The determination of lafine is not disturbed by the presence of the short 6 kDa form·。

产品特点：Detection level 878 pg/ml。

其他： 荷兰Hycult Biotech的宗旨和策略是着重于天然免疫相关疾病的研究，为人类生活质量的提高谋福利。作为免疫学和细胞生物学领域的领导者，特别是天然免疫、炎症反应和细胞损伤等相关领域的领先者，Hycult Biotech公司不仅研发这些用于生物科学研究的产品，同时也致力于将这些科研产品转化为临床测试的研究。作为多家知名品牌的中国总代理以及中国区域代理，艾德科技与Hycult一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量。

保存建议：推荐4℃保存，有效期一年。

定购信息： 

产品名称	规格	操作手册	货号	询价
弹力素(人)ELISA试剂盒	1*96det.	PDF	C-HK318-01
弹力素(人)ELISA试剂盒	2*96det.	PDF	C-HK318-02
【rIL 3 】重组大鼠白介素-3	5 ug	PDF	C-YT-383-1
【rIL 3 】重组大鼠白介素-3	20ug	PDF	C-YT-383-2
【rIL 3 】重组大鼠白介素-3	1 mg	PDF	C-YT-383-3

表观遗传学产品全面解决方案列下： 

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化试剂盒(定性分析)	48 次	PDF	A-P-1011-48
通用DNA甲基化试剂盒(定性分析)	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

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