SLPI(人)ELISA试剂盒 货号: C-HK316-02
SLPI, Human, ELISA kit
背景资料:The human SLPI ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3·5 hours· The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are captured by a solid bound specific antibody· Biotinylated tracer antibody will bind to captured human SLPI· Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody· Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of citric acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human SLPI standards (log)· The human SLPI concentration of samples, which are run concurrently with the standards, can be determined from the standard curve·。
产品描述:Secretory leukocyte proteinase inhibitor (SLPI; also known as antileukoprotease or ALP) is a 11·7 kDa cationic inhibitor of neutrophil elastase and to a lesser extent of cathepsin G· It is locally produced by epithelial cells in the lung, skin and other organs and by PMN and (in mice) by macrophages· In addition to its proteinase inhibitory properties that may serve to protect against proteolytic injury, it was recently shown that SLPI also displays several other functions such as antimicrobial and anti-inflammatory activities· These appear to be independent of its ability to inhibit PMN serine proteinases· SLPI has also been demonstrated to display antibacterial and antifungal activity at concentrations in which SLPI is present in mucosal secretions including those of the lung· Another possible role for SLPI is inhibition of protein disulphide isomerase that is considered essential for invasion of a cell by the Human Immunodeficiency Virus (HIV)· Serum/plasma and urine of healthy individuals contains 50-80 ng/ml and 4 ng/ml respectively· Sputum of healthy individuals contains approximately 66 microgram/milliliter。
产品特点:Detection level 20 pg/ml。
其他: 荷兰Hycult Biotech的宗旨和策略是着重于天然免疫相关疾病的研究,为人类生活质量的提高谋福利。作为免疫学和细胞生物学领域的领导者,特别是天然免疫、炎症反应和细胞损伤等相关领域的领先者,Hycult Biotech公司不仅研发这些用于生物科学研究的产品,同时也致力于将这些科研产品转化为临床测试的研究。作为多家知名品牌的中国总代理以及中国区域代理,艾德科技与Hycult一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量。
保存建议:推荐4℃保存,有效期一年。
定购信息:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
SLPI(人)ELISA试剂盒 |
1 x 96 det. |
PDF |
C-HK316-01 |
|
SLPI(人)ELISA试剂盒 |
2 x 96 det. |
PDF |
C-HK316-02 |
 |
【rIL 3 】重组大鼠白介素-3 |
5 ug |
PDF |
C-YT-383-1 |
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【rIL 3 】重组大鼠白介素-3 |
20ug |
PDF |
C-YT-383-2 |
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【rIL 3 】重组大鼠白介素-3 |
1 mg |
PDF |
C-YT-383-3 |
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