IL-8(人)ELISA试剂盒 货号:C-HK310-02
IL-8, Human, ELISA kit
背景资料:The human IL-8 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 4·5 hours· The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are captured by a solid bound specific antibody· Biotinylated tracer antibody will bind to captured human IL-8· Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody· Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of citric acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human IL-8 standards (log)· The human IL-8 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve。
产品描述:Interleukin-8 (IL-8) belongs to the CXC chemokine family· This cytokine has a wide range of proinflammatory properties ranging from its main function as chemoattractant for neutrophils, basophils and a subpopulation of lymphocytes, to activation of neutrophils· This ELR motive (Glu-Leu-Arg motive in NH2 terminus) positive CXC chemokine is a potent activator of angiogenesis· In vivo, IL-8 is detected in conditions of inflammation· High levels of IL-8 are observed during septic episodes in seriously ill patients· Various observations strongly suggest a role for the CXC chemokines IL-8 and IP-10 in the regulation of angiogenic activity in cancer and in idiopathic pulmonary fibrosis· In vitro, multiple cell types can produce IL-8· The cytokine can be rapidly detected after activation of cells with a wide variety of activators; endotoxin is a potent inducer of IL-8 release by monocytes and endothelial cells· The human IL-8 ELISA kit shows cross reactivity with most old world monkeys。
产品特点:Detection level 5 pg/ml。
其他: 荷兰Hycult Biotech的宗旨和策略是着重于天然免疫相关疾病的研究,为人类生活质量的提高谋福利。作为免疫学和细胞生物学领域的领导者,特别是天然免疫、炎症反应和细胞损伤等相关领域的领先者,Hycult Biotech公司不仅研发这些用于生物科学研究的产品,同时也致力于将这些科研产品转化为临床测试的研究。作为多家知名品牌的中国总代理以及中国区域代理,艾德科技与Hycult一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量。
保存建议:推荐4℃保存,有效期一年。
定购信息:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
IL-8(人)ELISA试剂盒 |
1 x 96 det. |
PDF |
C-HK310-01 |
|
IL-8(人)ELISA试剂盒 |
2 x 96 det. |
PDF |
C-HK310-02 |
|
【rIL 3 】重组大鼠白介素-3 |
5 ug |
PDF |
C-YT-383-1 |
|
【rIL 3 】重组大鼠白介素-3 |
20ug |
PDF |
C-YT-383-2 |
|
【rIL 3 】重组大鼠白介素-3 |
1 mg |
PDF |
C-YT-383-3 |
|
表观遗传学产品全面解决方案列下:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
 |
DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1037-96 |
|
DNA羟甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1036-48 |
 |
DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1036-96 |
|
DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
 |
DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
|
DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
 |
DNA甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1034-96 |
|
通用DNA甲基化试剂盒(定性分析) |
48 次 |
PDF |
A-P-1011-48 |
 |
通用DNA甲基化试剂盒(定性分析) |
96 次 |
PDF |
A-P-1011-96 |
|
超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
 |
超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
 |
总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
|
推荐阅读:
关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。 |