sTNF-RII(人)ELISA试剂盒                                 货号：C-HK302-02

sTNF-RII, Human, ELISA kit

背景资料：The sTNF-RII ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 4·5 hours· The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are captured by a solid bound specific antibody· Biotinylated tracer antibody will bind to captured sTNF-RII· Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody· Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of citric acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the sTNF-RII standards (log)· The sTNF-RII concentration of samples, which are run concurrently with the standards, can be determined from the standard curve。

产品描述：Tumor Necrosis Factor is a pleiotropic inflammatory cytokine· On almost all cells receptors for TNF have been detected· TNF-Receptors (TNF-R) were originally designated as TNF-binding proteins· These TNF-binding proteins present in urine and plasma were later found to be the extra-cellular part of the membrane bound cell signalling receptor for TNF-alpha and TNF-beta· Two types of TNF-Receptors (molecules belonging to the family of nerve growth receptor, CD27, CD40 etc) have been identified: TNF-RI (p55/p60), sCD120a and TNF-RII (p75/p80), sCD120b· The molecules share substantial identity in their extracellular part although the intra-cellular parts are structurally unrelated· This difference is reflected by the difference in function of the two receptors· Activation of various cell types leads to proteolytic cleavage of TNF-R resulting in soluble (extracellular part) TNF-R· Soluble TNF-R (sTNF-R) are considered to play a physiological role in TNF binding and inactivation, although also other functions such as stabilization of TNF in circulation have been mentioned· Detection of sTNF-R offers a means of investigating activation of the immune system both in vitro and in vivo· In plasma of healthy individuals approx· 1 - 4 ng/ml of sTNF-R of each type are present· The ELISA kit is also useful for quantitation of Rhesus monkey and Baboon sTNF-RII in plasma, serum and tissue culture media。

产品特点：Detection level 25 pg/ml。

其他： 荷兰Hycult Biotech的宗旨和策略是着重于天然免疫相关疾病的研究，为人类生活质量的提高谋福利。作为免疫学和细胞生物学领域的领导者，特别是天然免疫、炎症反应和细胞损伤等相关领域的领先者，Hycult Biotech公司不仅研发这些用于生物科学研究的产品，同时也致力于将这些科研产品转化为临床测试的研究。作为多家知名品牌的中国总代理以及中国区域代理，艾德科技与Hycult一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量。

保存建议：推荐4℃保存，有效期一年。

定购信息： 

产品名称	规格	操作手册	货号	询价
sTNF-RII(人)ELISA试剂盒	1 x 96 det.	PDF	C-HK302-01
sTNF-RII(人)ELISA试剂盒	2 x 96 det.	PDF	C-HK302-02
【rIL 3 】重组大鼠白介素-3	5 ug	PDF	C-YT-383-1
【rIL 3 】重组大鼠白介素-3	20ug	PDF	C-YT-383-2
【rIL 3 】重组大鼠白介素-3	1 mg	PDF	C-YT-383-3

表观遗传学产品全面解决方案列下： 

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化试剂盒(定性分析)	48 次	PDF	A-P-1011-48
通用DNA甲基化试剂盒(定性分析)	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

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