LAL Chromogenic Endpoint分析系统 货号:C-HIT302
LAL Chromogenic Endpoint assay
背景资料:The LAL assay is an enzyme-based assay with a working time of 45 minutes· The efficient format of 3 plates with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are incubated with LAL reagent· The enzymatic reaction, triggered by endotoxin, will cause a yellow color to develop upon cleavage of the chromophore, p-nitroaniline· The enzymatic reaction is stopped by the addition of acetic acid· The absorbance at 405 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the E· coli standards (log)· The endotoxin concentration of samples, which are run concurrently with the standards, can be determined from the standard curve。
产品描述:Bacterial endotoxin, like lipopolysaccharide (LPS), is a fever-producing by-product of gram-negative bacteria commonly known as pyrogen· The principle of the test is based on the fact that bacteria cause intravascular coagulation in the American horseshoe crab, Limulus polyphemus· The agent responsible for the clotting phenomena resided in the crab’s amoebocytes, or circulating blood cells, and that pyrogen (bacterial endotoxin) triggered the turbidity and gel-forming reaction enzymatically· Thus, endotoxin causes an opacity and gelation in Limulus amebocyte lysate (LAL), which is based on an enzymatic reaction· The simplicity and economy of the LAL chromogenic endpoint assay encourages the testing of various biologicals (including sera), devices, (air)filters and tissue culture medium for the presence of harmful levels of endotoxin。
产品特点:The kit has a minimum detection limit of 0·04 EU/ml。
其他: 荷兰Hycult Biotech的宗旨和策略是着重于天然免疫相关疾病的研究,为人类生活质量的提高谋福利。作为免疫学和细胞生物学领域的领导者,特别是天然免疫、炎症反应和细胞损伤等相关领域的领先者,Hycult Biotech公司不仅研发这些用于生物科学研究的产品,同时也致力于将这些科研产品转化为临床测试的研究。作为多家知名品牌的中国总代理以及中国区域代理,艾德科技与Hycult一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量。
保存建议:推荐4℃保存,有效期一年。
定购信息:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
LAL Chromogenic Endpoint分析系统 |
3*96det. |
PDF |
C-HIT302 |
 |
【rIL 3 】重组大鼠白介素-3 |
5 ug |
PDF |
C-YT-383-1 |
|
【rIL 3 】重组大鼠白介素-3 |
20ug |
PDF |
C-YT-383-2 |
|
【rIL 3 】重组大鼠白介素-3 |
1 mg |
PDF |
C-YT-383-3 |
|
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