1· Discrete clusters and high call rates for accurate and reproducible allelic discrimination;All 168 human genomic DNA samples were accurately genotyped using the ABI sequence detection system (SDS) version 2.3 software (autocaller confidence level 95%). A total of 168 human genomic DNA samples were successfully genotyped along with 24 no-template controls using an ATP1B3 SNP genotyping assay on the ABI 7900HT real-time PCR system. Reactions were performed in 5 μl volumes with KAPA PROBE FAST qPCR Master Mix, human genomic DNA (10 ng per reaction), 200 nM of each primer and 200 nM of each hydrolysis probe (Allele X – FAM/ BHQ®-1, Allele Y – VIC/ BHQ®-1) using the following standard cycling protocol: 95 ºC for 10 min followed by 40 cycles of 95 ºC, 15 sec; 60 ºC, 60 sec
2·Fast, high performance five-color multiplexed qPCR;Highly reproducible and efficient results for all 5 amplicons across a 5 point dilution series of human genomic DNA were obtained when assayed in penta-plex using a fast cycling protocol. Standard curves were generated using 4-fold dilutions of human genomic DNA (0.39 – 100 ng per reaction) tested in triplicate using the Corbett Rotor-Gene™ 6000 HRM real-time rotary analyzer. Reactions were performed in 20 μl volumes with KAPA PROBE FAST qPCR Master Mix, human genomic DNA, 200 nM of each primer and 200 nM of each hydrolysis probe (ACTB - FAM™/BHQ®-1, ERBB2 - CAL Fluor® Gold 540/ BHQ®-1, ERBB3 - CAL Fluor® Orange 560/ BHQ®-2, EGFR - CAL Fluor® Red 610/ BHQ®-2, ACTG1 - Quasar® 705/ BHQ®-2) using the following fast cycling protocol: 95 ºC for 3 min followed by 40 cycles of 95 ºC, 15 sec; 60 ºC, 15 sec
3·Precise and highly reproducible discrimination;Excellent reproducibility and discrimination across samples of low copy number with similar abundance levels was achieved. Reactions were performed in 20 μl volumes with KAPA PROBE FAST qPCR Master Mix, human genomic DNA (1.5-fold dilutions over a 89 - 450 copies per reaction range), 200 nM of each primer and 200 nM of hApoB100 (FAM/ BHQ®-1) hydrolysis probe using a fast cycling protocol (95 ºC for 3 min followed by 40 cycles of 95 ºC, 3 sec; 60 ºC, 20 sec) on the Corbett Rotor-Gene ™ 6000 HRM real-time rotary analyzer
4.Broad linear dynamic range of up to 10 orders of magnitude using a fast cycling protocol;
5.High performance with both standard and fast cycling protocols;
6.Excellent benchtop stability.
保存建议:在接收到艾德寄出的试剂盒后请按照说明书建议,使用不同的保存条件或温度来保存试剂盒内组分
定购信息:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
ABI PrismTMPROBE快速定量试剂盒 |
1 ml |
PDF |
A-KK4705 |
 |
ABI PrismTMPROBE快速定量试剂盒 |
5 ml |
PDF |
A-KK4706 |
|
ABI PrismTMPROBE快速定量试剂盒 |
10 ml |
PDF |
A-KK4707 |
|
Bio-Rad SYBR快速定量试剂盒 |
1 ml |
PDF |
A-KK4606 |
|
Bio-Rad SYBR快速定量试剂盒 |
5 ml |
PDF |
A-KK4607 |
|
Bio-Rad SYBR快速定量试剂盒 |
10 ml |
PDF |
A-KK4608 |
|
ABI Prism SYBR快速定量试剂盒 |
1 mL |
PDF |
A-KK4603 |
|
ABI Prism SYBR快速定量试剂盒 |
5 mL |
PDF |
A-KK4604 |
|
ABI Prism SYBR快速定量试剂盒 |
10 mL |
PDF |
A-KK4605 |
|
Paq5000 Hotstart PCR Master Mix |
400 次 |
PDF |
A-600872 |
|
Paq5000 Hotstart PCR Master Mix |
100 次 |
PDF |
A-600870 |
|
植物DNA小量提取试剂盒 |
50 次 |
PDF |
A-DH813-01 |
|
植物DNA小量提取试剂盒 |
200 次 |
PDF |
A-DH813-02 |
|
凝胶DNA纯化回收试剂盒 |
100 次 |
PDF |
A-D2110-01 |
|
凝胶DNA纯化回收试剂盒 |
200 次 |
PDF |
A-D2110-02 |
|
凝胶DNA纯化回收试剂盒 |
500 次 |
PDF |
A-D2110-03 |
|
凝胶DNA纯化回收试剂盒 |
1000次 |
PDF |
A-D2110-04 |
|
胶自动纯化回收试剂盒(磁珠法) |
150 次 |
PDF |
A-DM136-01 |
|
胶自动纯化回收试剂盒(磁珠法) |
500 次 |
PDF |
A-DM136-02 |
|
总RNA自动化富集纯化mRNA试剂盒(磁珠法) |
30 次 |
PDF |
A-RM911-01 |
|
植物自动化DNA大量提取试剂盒(磁珠法) |
50 次 |
PDF |
A-DM311-01 |
|
组织切片DNA极速提取试剂盒 |
100 次 |
PDF |
A-P-1003-100 |
|
表观遗传学产品全面解决方案列下:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
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DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1037-96 |
|
DNA羟甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1036-48 |
 |
DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1036-96 |
|
DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
 |
DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
|
DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
 |
DNA甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1034-96 |
|
通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
 |
通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
 |
超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
 |
总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
|
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