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DNA |
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| DNA>常用PCR相关/DNA Clean-up |
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A&D-96 DNA Clean & Concentrator™-5 (D4023 D4024) |
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ZR-96 DNA Clean & Concentrator™-5
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Simple, rapid recovery of ultra-pure DNA from PCR, endonuclease digestions, “crude” DNA preps., etc. Perfect for post-RT cDNA clean-up and purification of sequencing-ready DNA from M13 phage infected culture supernatants. |
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Column and deep-well filtration plate designs allow DNA to be eluted at high concentrations into minimal volumes of water or TE buffer. |
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Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc. |
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Format: 96-well Plate |
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Sample Sources: DNA from PCR, restriction endonuclease digestions, “crude” DNA preparations, etc. |
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DNA Size Limits: 50 bp to 23 kb |
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DNA Recovery: Typically, ≤5 µg total DNA can be eluted with ≥6 µl water or low salt elution buffer (≥10 µl for ZR-96 DCC™-5). For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
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DNA Purity: Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. |
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Detergent Tolerance: ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
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Equipment Needed: Centrifuge (with microplate carriers) |
Descriptions
Zymo Research’s ZR-96 DNA Clean & Concentrator-5™ (ZR-96 DCC™) provides a hassle-free method for the rapid, large-scale (96 sample) purification and concentration of high-quality DNA from PCR samples, endonuclease digestions, or “crude” plasmid preparations. Simply add the specially formulated DNA Binding Buffer to your samples and transfer to the wells of the supplied Zymo-Spin™ I-96 Plate. There is no need for organic denaturants or chloroform. Instead, the product features Fast-Spin column technology to yield high-quality, purified DNA in just minutes. DNA purified using the ZR-96 DCC™ is suitable for nucleotide sequencing, array analysis, PCR, nucleotide blotting, and restriction endonuclease digestion procedures.
The ZR-96 DCC™ employs a single-buffer system that allows for efficient DNA adsorption of up to 96 samples onto the matrix of the supplied Zymo-Spin™ I-96 Plate. The DNA is washed twice then eluted with a small volume of water into a 96-well Elution Plate. The entire DNA purification/concentration procedure typically takes about 20 minutes. The figure below shows a schematic highlighting the Zymo-Spin™ I-96 Plate design.
| Product |
Catalog No. |
Size |
Price(RMB) |
Protocol |
ZR-96 DNA Clean & Concentrator™-5
(deep well) |
D4023 |
2x96 Preps. |
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D4024 |
4x96 Preps. |
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