EndoCab(人)ELISA试剂盒                                             货号：E-HK504

EndoCab, Human, ELISA kit

背景资料：The EndoCab ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 2·5 hours· The efficient format of 1 plate with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are incubated in microtiter wells coated with equimolar amounts of endotoxin rough-lipopolysaccharides from four Gram-negative bacterial species, each comprised of a complete inner core, but lacking complete outer core or O-specific polysaccharide chain· These endotoxins, complexed with polymyxin B, constitute the solid phase antigen· During this incubation anti-endotoxin-core antibodies are captured by the solid phase antigen· Unbound material present in the sample is removed by wash璱ng· Peroxidase conjugated anti-human Ig(G or M or A) tracer antibody is added to the wells· If EndoCab antibodies were present in the sample, tracer antibodies will bind to the captured EndoCab antibodies· Excess tracer is removed by washing and substra璽e, tetramethylben瓃idine (TMB), is added to the wells· Color develops proportionally to the amount of anti endotoxin core antibodies present in the sample· The enzyme reaction is stopped by the addition of citric acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the LBP standards (log)· The human EndoCab antibody concentration of samples, which are run concurrently with the human LBP standards, can be determined from the human LBP standard curve··

产品描述：The EndoCab ELISA has been developed for determination of endotoxin core antibodies in human plasma or serum in patients or healthy individuals· Several studies show a consistent drop in postoperative levels of circulating anti-endotoxin core antibodies from the preoperative value· This drop has been interpreted as consumption of antibodies to endotoxin by systemic release of endotoxin· A hypothesis is that if the patients pre-operative EndoCab level is low, even moderately low, patients may not be able to cope with the efflux of endotoxin, which may have mild to severe clinical consequences· The assay is of interest for various experimental conditions ranging from in vitro LPS neutralization by plasma components to in vivo studies on kinetics of antibodies to endotoxin in health and diseases· The EndoCab standard median-units IgG, IgM and IgA (MU) are arbitrary and are based on medians of ranges for 1000 healthy adults in a particular locality· It has not been established whether normal EndoCab values vary by region, culture or race· Users should establish appropriate local statistical controls for their studies· It is advised that studies of any patient group should always be controlled by studies of appropriately selected contrasting clinical groups and/or healthy individuals recruited locally· EndoCab is a registered trademark· Used under license from the Scottish National Blood Transfusion Service··

产品特点：Detection level Minimum detection level IgG EndoCab antibodies is 0·13 GMU/ml· Minimum detection level IgM EndoCab antibodies is 0·05 MMU/ml· Minimum detection level IgA EndoCab antibodies is 0·16 AMU/ml·

保存建议：推荐4℃保存，有效期一年。

定购信息： 

产品名称	规格	操作手册	货号	询价
EndoCab(人)ELISA试剂盒	1 x 96 det	PDF	E-HK504-01
VisionBlue快速细胞活力分析试剂盒	500 次	PDF	C-K303-500

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化试剂盒(定性分析)	48 次	PDF	A-P-1011-48
通用DNA甲基化试剂盒(定性分析)	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

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