8-OHdG DNA损伤定量检测试剂盒(荧光法) 货号：A-P-6004
EpiQuik 8-OHdG DNA Damage Quantification Direct Kit (Fluorometric)
背景资料：8-hydroxy-2’-deoxyguanosine (8-OHdG or 8-oxo-dG) is an oxidized derivative of deoxyguanosine and is generated by hydroxyl radicals, singlet oxygen, and one-electron oxidants in cellular DNA. As a modified nucleoside base, 8-OHdG is considered important not only because of its abundance but also because of its mutagenic potential through G-to-T transversion mutations upon replication of DNA. 8-OHdG also participates in epigenetic regulation of gene activation/repression by inhibiting the binding affinity of MBD protein to the CpG sites of DNA. Currently, 8-OHdG is widely accepted as a sensitive marker of oxidative DNA damage and oxidative stress. Evidence shows that increased levels of 8-OHdG are closely correlated with exposure to harmful environmental factors such as ionizing radiation, industrial chemicals, air pollution, cigarette smoking, and cancer chemotherapy.
产品描述：The EpiQuik™ 8-OHdG DNA Damage Quantification Direct Kit (Fluorometric) contains all reagents necessary for the quantification of Oxidative DNA damage (8-OHdG). In this assay, DNA is bound to strip wells that are specifically treated to have a high DNA affinity. 8-OHdG is detected using capture and detection antibodies. The detected signal is enhanced and then quantified fluorometrically by reading the fluorescence in a fluorescence microplate reader The amount of 8-OHdG is proportional to the fluorescence intensity measured.
产品特点：
1·Fluorometric assay with easy-to-follow steps for convenience and speed. The entire procedure can be completed within 3 hours and 45 minutes.
2·High sensitivity, of which the detection limit can be as low as 1 pg of 8-OHdG.
3.High specificity by detecting only 8-OHdG without cross-reactivity to 8-OHdG analogues.
4.Direct detection of 8-OHdG using intact DNA, which eliminates interference from high molecular weight compounds, such as carbohydrates and proteins that are often seen in competitive 8-OHdG assays.
5.Detection accuracy is highly correlated with and close to HPLC or LC-MS analysis.
6.Highly convenient assay with direct use of DNA isolated from cells or tissues, without the need for DNA digestion or hydrolysis.
7.Universal positive and negative controls are included, which are suitable for quantifying 8-OHdG from any species.
8.Strip-well microplate format makes the assay flexible for manual or high throughput analysis.
9.Simple, reliable, and consistent assay conditions.
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分。
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| 8-OHdG DNA损伤定量检测试剂盒(荧光法) |
48 次 |
PDF |
A-P-6004-48 |
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| 8-OHdG DNA损伤定量检测试剂盒(荧光法) |
96 次 |
PDF |
A-P-6004-96 |
|
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