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  micro RNA极速提取试剂盒(A-R5301)
   
micro RNA极速提取试剂盒
 
快速——30分钟完成实验。
高效分离小RNA,同时分离总RNA
富集200nt以下的小RNA,增加其用于下游实验的浓度。
可用于miRNAsiRNAshRNAsnRNA分析。
 
 
Format: Spin Column
Sample Sources: RNA fragments (≥17 nucleotides), labeled RNA fragments, in vitro transcription products, etc.还适用于各种来源的样品。
microRNA Recovery: Typically, RNA is eluted into as little as 6-10 µl RNase-free water allowing for a highly concentrated sample. The RNA binding capacity of the supplied RB Columns is ~5 µg.
microRNA Purity: High quality microRNA (A260/A280 > 1.8, A260/A230 > 1.8) suitable for all downstream RNA-based manipulations.
microRNA Storage: microRNA is eluted with RNase-free water and can be stored at ≤ -70°C. The addition of RNase inhibitors is optional but highly recommended for prolonged storage.
Equipment Needed: Microcentrifuge for ex.eppendorf 5415c

Descriptions

micro RNA极速提取试剂盒 provides a simple and reliable method for the rapid preparation of up to ~5 μg (per prep.) of high-quality RT-PCR-ready, DNA-free RNA. This simple procedure is based on the use of a unique single-buffer system and Fast-Spin column technology. The procedure is easy: treat your RNA sample with DNase I, add binding buffer, adjust the conditions by adding ethanol, and the cleaned RNA is then concentrated into ≥6 μl of RNase-free water using the RB Column. RNA fragments (≥17 bases) can be safely treated and recovered using this kit. The result is highlyconcentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc. The purification procedure after the DNase treatment typically takes about 5 minutes.

 
 

Purification of RNA samples containing genomic and plasmid DNA by using the micro RNA极速提取试剂盒. (1) RNA/DNA sample purified without prior DNase treatment, (2) DNase treated, purified RNA sample.

 
 

Product Name Catalog No. Size Price(RMB) Protocol
micro RNA极速提取试剂盒 A-R5301 50 次 点击这里给我发消息
  A-R5302 200 次

RT-PCR相关产品全面解决方案列下:

产品名称 规格 操作手册 货号 询价
Thermo M-MLV 反转录酶 10000U PDF A-P6801 点击这里给我发消息
Thermo M-MLV 反转录酶 50000U PDF A-P6802  
Supermo III M-MLV 反转录酶 10000U PDF A-P6811 点击这里给我发消息
Supermo III M-MLV 反转录酶 50000U PDF A-P6812  
Super M-MLV 反转录酶 10000U PDF A-P6502 点击这里给我发消息
Super M-MLV 反转录酶 50000U PDF A-P6503  

推荐阅读:

关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化

 
 
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